AB109195

重组Anti-CHD3抗体[EPNCIR110A]

Anti-CHD3 antibody [EPNCIR110A]

RabMAb
Recombinant
KO Validated
20ul selling size
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(4 Publications)

Rabbit Recombinant Monoclonal CHD3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 4 publications.

查看别名

Chromodomain-helicase-DNA-binding protein 3, CHD-3, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.

Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CHD3 knockout HAP1 cell lysate (20 μg)
Lane 3 : Raji cell lysate (20 μg)
Lane 4 : Y79 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

Predicted band size: 226 kDa

false

Unknown

Western blot - Anti-CHD3 antibody [EPNCIR110A] (AB109195)

All lanes:

Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Predicted band size: 226 kDa

Observed band size: 260 kDa

false

不同偶联物与剂型 (10)

Carrier free

Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free
660 APC

APC Anti-CHD3 antibody [EPNCIR110A]
HRP

HRP Anti-CHD3 antibody [EPNCIR110A]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CHD3 antibody [EPNCIR110A]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CHD3 antibody [EPNCIR110A]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CHD3 antibody [EPNCIR110A]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CHD3 antibody [EPNCIR110A]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CHD3 antibody [EPNCIR110A]
578 PE

PE Anti-CHD3 antibody [EPNCIR110A]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CHD3 antibody [EPNCIR110A]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPNCIR110A

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Collaborations
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CHD3 also known as Chromodomain Helicase DNA Binding Protein 3 is a protein involved in transcription regulation. The protein has a molecular mass of approximately 220 kDa. CHD3 is a member of the chromatin remodeler family and is expressed widely throughout various tissues. It contains dual chromodomains a helicase/ATPase domain and a DNA-binding domain enabling it to remodel chromatin structure by altering nucleosome positioning on DNA.

Biological function summary

CHD3 plays a significant role in modifying chromatin architecture and regulating the accessibility of the DNA to transcription factors. It functions as part of the NuRD (Nucleosome Remodeling and Deacetylase) complex which combines the features of ATP-dependent chromatin remodeling and histone deacetylase activity. CHD3 helps remodel nucleosomes and remove acetyl groups from histones which leads to chromatin condensation and transcriptional repression. Its activity influences genes involved in development differentiation and cell cycle control.

Pathways

CHD3's function integrates into pathways that control gene expression and DNA repair. It plays a role in the transcriptional regulation pathway working alongside proteins like HDAC1 and NuRD components. Additionally CHD3 participates in the DNA damage response cooperating with proteins such as CHD4 and RBBP4 to ensure genomic stability. These interactions and functions place CHD3 at a strategic position within cellular mechanisms maintaining transcription fidelity and genomic integrity.

CHD3 associations with cancer and developmental disorders stand out. Abnormal expression or mutations in CHD3 can contribute to tumorigenesis as it can affect the expression and repression of oncogenes and tumor suppressor genes. Furthermore CHD3 mutations have been linked to Snijders Blok-Campeau syndrome a neurodevelopmental disorder. The interaction between CHD3 and other proteins like HDAC1 and CHD4 plays a critical role in the manifestation of these conditions influencing pathways that drive disease progression and phenotype expression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed : 28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666, PubMed : 30397230, PubMed : 9804427). Involved in transcriptional repression as part of the NuRD complex (PubMed : 27068747). Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity (PubMed : 17626165).

See full target information CHD3

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 626:881-890 PubMed38297124

2024

An epigenetic barrier sets the timing of human neuronal maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Ciceri,Arianna Baggiolini,Hyein S Cho,Meghana Kshirsagar,Silvia Benito-Kwiecinski,Ryan M Walsh,Kelly A Aromolaran,Alberto J Gonzalez-Hernandez,Hermany Munguba,So Yeon Koo,Nan Xu,Kaylin J Sevilla,Peter A Goldstein,Joshua Levitz,Christina S Leslie,Richard P Koche,Lorenz Studer

Genetics in medicine : official journal of the American College of Medical Genetics 24:1283-1296 PubMed35346573

2022

Inherited variants in CHD3 show variable expressivity in Snijders Blok-Campeau syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jet van der Spek,Joery den Hoed,Lot Snijders Blok,Alexander J M Dingemans,Dick Schijven,Christoffer Nellaker,Hanka Venselaar,Galuh D N Astuti,Tahsin Stefan Barakat,E Martina Bebin,Stefanie Beck-Wödl,Gea Beunders,Natasha J Brown,Theresa Brunet,Han G Brunner,Philippe M Campeau,Goran Čuturilo,Christian Gilissen,Tobias B Haack,Irina Hüning,Ralf A Husain,Benjamin Kamien,Sze Chern Lim,Luca Lovrecic,Janine Magg,Ales Maver,Valancy Miranda,Danielle C Monteil,Charlotte W Ockeloen,Lynn S Pais,Vasilica Plaiasu,Laura Raiti,Christopher Richmond,Angelika Rieß,Eva M C Schwaibold,Marleen E H Simon,Stephanie Spranger,Tiong Yang Tan,Michelle L Thompson,Bert B A de Vries,Ella J Wilkins,Marjolein H Willemsen,Clyde Francks,Lisenka E L M Vissers,Simon E Fisher,Tjitske Kleefstra

PloS one 15:e0235799 PubMed32658897

2020

The chromatin-remodeling enzyme CHD3 plays a role in embryonic viability but is dispensable for early vascular development.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xie,Siqi Gao,Christopher Schafer,Sarah Colijn,Vijay Muthukumar,Courtney T Griffin

The Journal of biological chemistry 293:15439-15454 PubMed30082317

2018

The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Rodríguez-Castañeda,Roza Berhanu Lemma,Ignacio Cuervo,Mads Bengtsen,Lisa Marie Moen,Marit Ledsaak,Ragnhild Eskeland,Odd Stokke Gabrielsen

View all publications

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重组Anti-CHD3抗体[EPNCIR110A]