重组Anti-CHD3抗体[EPNCIR110] (ab134125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR110] to CHD3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CHD3抗体[EPNCIR110]
参阅全部 CHD3 一抗 -
描述
兔单克隆抗体[EPNCIR110] to CHD3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse CHD3.
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阳性对照
- Mouse brain lysate, Raw264.7 cell lysate, NIH 3T3 cell lysate and cells, Mouse spleen tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR110 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 234 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 234 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 216848 Mouse
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别名
- 226 antibody
- 2600010P09Rik antibody
- AF020312 antibody
see all
图片
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All lanes : Anti-CHD3 antibody [EPNCIR110] (ab134125) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 234 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110] (ab134125)
Immunohistochemical analysis of paraffin embedded Mouse spleen tissue labelling CHD3 with ab134125 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of 3T3 cells labelling CHD3 with ab134125 at 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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