Anti-CGRP 抗体

• ELISA

Supplier Data

ELISA - Anti-CGRP antibody (AB36001)

Goat anti Rat Calcitonin Gene-Related Peptide ELISA. Synthetic Rat Calcitonin Gene-Related Peptide detected using ab36001 as the capture reagent and Donkey anti Sheep/Goat IgG : HRP as the detection reagent.

• IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (AB36001)

CGRP immunohistochemistry using Anti-CGRP antibody ab36001. Publication image and figure legend from Mathews, M. A., Murray, A., et al. 2015, , PLoS One, PubMed : 26422206.

Immunohistochemical staining and retrograde tracing of the EVN.VN : vestibular nucleus; G7n : genu of seventh cranial nerve (facial nerve); 6n : sixth cranial nerve nucleus (abducens nucleus). (A&C) Immunohistochemical staining for CGRP (n = 7) and ChAT (n = 4) respectively in transversely sectioned mouse brainstem. The EVN is located dorsolateral to genu of seventh nerve (box). Neurons in the abducens and vestibular nucleus were also labelled. (B&D) Higher power visualization of vestibular efferent nucleus. Arrowheads indicate CGRP and ChAT immuno-positive cells respectively. (E&F) Fluorogold injection into the posterior canal of ChAT : : tdTomato mouse strain under low and high power respectively (n = 4). Arrowheads indicate co-localization (yellow) of fluorogold (green) and tdTomato (red), confirming the location of vestibular efferent nucleus.

• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (AB36001)

CGRP immunohistochemistry (frozen sections) using Anti-CGRP antibody ab36001. Publication image and figure legend from Ma, S. B., Xian, H., et al., 2020, Mol Brain PubMed 33228784.

CCR2 is widely expressed in DRG neurons and displays upregulation following peripheral inflammation. a, b Typical examples and quantitative summary from dual immunofluorescence experiments showing CCR2 immunoreactivity IB4-binding non-peptidergic DRG neurons in the naïve and CFA-inflamed mice. c, d Immunofluorescence experiments and quantitative summary showing the coexistence of CCR2 immunoreactivity in CGRP-expressing peptidergic DRG neurons in naïve mice and its upregulation in CFA-inflamed mice. e, f Immunofluorescence staining and quantitative summary showing the colocalization of CCR2 immunoreactivity with NF200-positive large-diameter DRG neurons in naïve mice and no significant change in expression level after CFA inflammation. Note that in CFA-inflamed group, ipsilateral L3/L4 DRGs were obtained and analyzed at 24 h after intraplantar CFA injection. Arrows indicate the co-expressing cells. **P < 0.01. Two-Way ANOVA followed by post-hoc Bonferroni test (n = 4 mice/group). Data are expressed as mean ± SEM