Anti-CGRP抗体[4901] (ab81887)
![Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.cn/ps/products/81/ab81887/Images/ab81887-96924-anti-cgrp-antibody-4901-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)")
Key features and details
- Mouse monoclonal [4901] to CGRP
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG2a
概述
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产品名称
Anti-CGRP抗体[4901]
参阅全部 CGRP 一抗 -
描述
小鼠单克隆抗体[4901] to CGRP -
宿主
Mouse -
特异性
ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP). -
经测试应用
适用于: ICC/IF, IHC-Fr, Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Mouse, Rat, Guinea pig, Human
预测可用于: Mammals
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免疫原
Full length protein corresponding to Rat CGRP aa 1 to the C-terminus.
Database link: P01256 -
阳性对照
- Dorsal root ganglia
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue. -
克隆
单克隆 -
克隆编号
4901 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/50 - 1/100.
|
| IHC-Fr | (4) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration. PubMed: 24430801
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. PubMed: 24430801 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for WB.
靶标
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相关性
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
细胞定位
Endoplasmic reticulum and Secreted -
数据库链接
- Entrez Gene: 796 Human
- Entrez Gene: 797 Human
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- Omim: 114160 Human
see all -
别名
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (153)
ab81887 被引用在 153 文献中.
- Wu H et al. Temporal alterations of pituitary adenylate cyclase activating polypeptide and its receptors in a rat model induced by recurrent chemical stimulations: Relevant to chronic migraine. Mol Pain 19:17448069231152129 (2023). PubMed: 36604785
- Belinskaia M et al. Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins. Int J Mol Sci 24:N/A (2023). PubMed: 36674850
- Yang OJ et al. Evaluating the transduction efficiency of systemically delivered AAV vectors in the rat nervous system. Front Neurosci 17:1001007 (2023). PubMed: 36755734
- Arthurs JW et al. Activation of Parabrachial Tachykinin 1 Neurons Counteracts Some Behaviors Mediated by Parabrachial Calcitonin Gene-related Peptide Neurons. Neuroscience 517:105-116 (2023). PubMed: 36898496
- Nimbalkar S et al. Development of a functional human induced pluripotent stem cell-derived nociceptor MEA system as a pain model for analgesic drug testing. Front Cell Dev Biol 11:1011145 (2023). PubMed: 36936691