重组Anti-cGMP抗体[EPR10355] (ab169753)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10355] to cGMP
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Related conjugates and formulations
概述
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产品名称
Anti-cGMP抗体[EPR10355]
参阅全部 cGMP 一抗 -
描述
兔单克隆抗体[EPR10355] to cGMP -
宿主
Rabbit -
经测试应用
适用于: Competitive ELISAmore details
不适用于: Flow Cyt,ICC/IF,IHC-P,IP or WB -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to cGMP conjugated to keyhole limpet haemocyanin.
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常规说明
The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR10355 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use a concentration of 0.1 - 4 µg/ml.
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说明 |
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Competitive ELISA
Use a concentration of 0.1 - 4 µg/ml. |
靶标
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相关性
Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
别名
- Cyclic GMP antibody
- Cyclic guanosine monophosphate antibody
- Guanosine 3 5 Cyclic Monophosphate antibody
图片
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2 µg/ml of Goat anti-rabbit IgG (50 µl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 µg/ml of ab169753 (50 µl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 µl/well) were added along with 25 µl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
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2µg/ml of goat-anti-rabbit IgG (50µl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2µg/ml of ab169753 (50 µl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 µl / well each) were added, respectively. Finally 25 µl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab169753 尚未被引用在任何文献中。