AB92738

重组Anti-CGA/hCG alpha抗体[EP3373]

Anti-CGA/hCG alpha antibody [EP3373]

BOND RX™ Validated
RabMAb
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Monoclonal GLHA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

查看别名

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CGA/hCG alpha with ab92738 at 1/600 dilution (0.87 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/2000 dilution

Lane 1:

Western blot - Anti-PKR antibody [EPR19374] (<a href='/products/primary-antibodies/pkr-antibody-epr19374-ab184257'>ab184257</a>) at 1/1000 dilution

Lane 1:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate

Lane 1:

bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate treated with Hydroxyurea (10mM 24h)

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Carrier free

Anti-CGA/hCG alpha antibody [EP3373] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3373

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "For unpurified, use 1/100 - 1/250." } } }

style

left-column

产品详情

This product has switched from a hybridoma to recombinant production method on 2nd August 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CGA also known as the hCG alpha subunit is a glycoprotein hormone free alpha subunit. Its molecular mass is approximately 15-22 kDa. It is a part of the human chorionic gonadotropin (hCG) protein. It is expressed in the placenta and various neoplastic tissues. CGA along with the beta subunit forms the complete structure of hCG which plays a significant role in signaling for the reproductive processes.

Biological function summary

This alpha subunit plays a fundamental role in forming the heterodimer with different beta subunits of hCG TSH LH and FSH resulting in various glycoprotein hormones. These hormones are essential for maintaining pregnancy fetal development and regulating the menstrual cycle. The CGA protein is key to the activity of the hCG protein which significantly impacts fetal and trophoblastic growth during pregnancy. The heterodimeric complex to which it contributes assigns it unique biological roles that differ depending on the linked beta subunit.

Pathways

The CGA subunit functions within the reproductive hormone signaling pathway. The uniform alpha subunit pairs with distinct beta subunits such as LH beta or FSH beta influencing different signaling pathways. It plays a vital part in the hormonal regulation pathway modulating endocrine and reproductive processes. The related proteins include the beta subunits like hCG beta LH beta and FSH beta which pair with CGA to perform distinct biological functions in different tissues.

CGA's expression relates to trophoblastic diseases and certain types of cancer such as choriocarcinoma. Aberrant expression or regulation can be seen in these conditions linking it to tumorigenesis and abnormal reproductive disorders. Choriocarcinoma significantly involves hCG protein and its subunits where CGA influences the progression by contributing to this hormone's abnormal activity. Elevated levels of CGA can correlate with the progression of these diseases serving as a clinical marker for diagnosis.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

See full target information CGA

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

Pituitary 18:31-41 PubMed24402129

2014

Expression of the pituitary stem/progenitor marker GFRα2 in human pituitary adenomas and normal pituitary.

Applications

Unspecified application

Species

Unspecified reactive species

Nestoras Mathioudakis,Ram Sundaresh,Alexandra Larsen,William Ruff,Jennifer Schiller,Hugo Guerrero-Cázares,Peter Burger,Roberto Salvatori,Alfredo Quiñones-Hinojosa

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CGA/hCG alpha抗体[EP3373]