Anti-Ceruloplasmin抗体(ab48614)
Key features and details
- Rabbit polyclonal to Ceruloplasmin
- Suitable for: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Ceruloplasmin抗体
参阅全部 Ceruloplasmin 一抗 -
描述
兔多克隆抗体to Ceruloplasmin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFrmore details -
种属反应性
与反应: Human -
免疫原
Human ceruloplasmin purified from human plasma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48614于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| RIA |
Use at an assay dependent concentration.
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| EIA |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
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| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
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| IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
|
RIA
Use at an assay dependent concentration. |
|
EIA
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. |
|
IHC-FoFr
Use at an assay dependent concentration. |
靶标
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功能
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
序列相似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1356 Human
- Omim: 117700 Human
- SwissProt: P00450 Human
- Unigene: 558314 Human
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别名
- CERU_HUMAN antibody
- Ceruloplasmin antibody
- CP 2 antibody
see all
图片
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Western blot - Anti-Ceruloplasmin antibody (ab48614)Anti-Ceruloplasmin antibody (ab48614) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 122,148 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Ceruloplasmin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted (148 kDa). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ceruloplasmin antibody (ab48614)Ab48614 staining human tonsil. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab48614 被引用在 13 文献中.
- Jacquemin G et al. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. Elife 11:N/A (2022). PubMed: 35543624
- Shin HJ et al. Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death. Antioxidants (Basel) 10:N/A (2021). PubMed: 33445746
- Madani AY et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
- Tsai YM et al. Loss of miR-145-5p Causes Ceruloplasmin Interference with PHD-Iron Axis and HIF-2a Stabilization in Lung Adenocarcinoma-Mediated Angiogenesis. Int J Mol Sci 21:N/A (2020). PubMed: 32708433
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611