Anti-CERT抗体(ab72536)
Key features and details
- Rabbit polyclonal to CERT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CERT抗体
参阅全部 CERT 一抗 -
描述
兔多克隆抗体to CERT -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide within Human CERT aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005704.1
Database link: Q9Y5P4-1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72536于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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| IP |
Use at 1-4 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
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IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
May mediate the intracellular trafficking of ceramide in a non-vesicular manner. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 PH domain.
Contains 1 START domain. -
结构域
The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB. -
翻译后修饰
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. -
细胞定位
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 286797 Cow
- Entrez Gene: 10087 Human
- Entrez Gene: 100173502 Orangutan
- Entrez Gene: 432007 Xenopus laevis
- Entrez Gene: 550627 Zebrafish
- Entrez Gene: 796455 Zebrafish
- Omim: 604677 Human
- SwissProt: Q9GKI7 Cow
see all -
别名
- Alpha 3 type IV collagen binding protein antibody
- C43BP_HUMAN antibody
- Ceramide transfer protein antibody
see all
图片
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Western blot - Anti-CERT antibody (ab72536)All lanes : Anti-CERT antibody (ab72536) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 100 kDa, 28 kDa, 60 kDa, 70 kDa (possible isoform). We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-CERT antibody (ab72536)Detection of Human CERT by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab72536 被引用在 14 文献中.
- Shimasaki K et al. Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum. Int J Mol Sci 23:N/A (2022). PubMed: 35409383
- Goto A et al. Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide. iScience 25:104624 (2022). PubMed: 35800758
- Goto A et al. Involvement of a Cluster of Basic Amino Acids in Phosphorylation-Dependent Functional Repression of the Ceramide Transport Protein CERT. Int J Mol Sci 23:N/A (2022). PubMed: 35955719
- Benedyk TH et al. Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT. J Biol Chem 298:102589 (2022). PubMed: 36243114
- Barman B et al. VAP-A and its binding partner CERT drive biogenesis of RNA-containing extracellular vesicles at ER membrane contact sites. Dev Cell 57:974-994.e8 (2022). PubMed: 35421371