Anti-CERKL抗体(ab222828)
Key features and details
- Rabbit polyclonal to CERKL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CERKL抗体
参阅全部 CERKL 一抗 -
描述
兔多克隆抗体to CERKL -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human CERKL aa 1 to the C-terminus.
Database link: Q49MI3 -
阳性对照
- WB: Mouse lung tissue lysate. IHC-P: Human prostate cancer and pancreatic tissues. ICC/IF: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
>95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| WB |
1/1000 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 20,25,43,48,53,58,60,63 kDa).
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| 说明 |
|---|
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IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
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WB
1/1000 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 20,25,43,48,53,58,60,63 kDa). |
靶标
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功能
Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions. -
组织特异性
Isoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver. -
疾病相关
Defects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive. -
序列相似性
Contains 1 DAGKc domain. -
发展阶段
Expressed in fetal lung, kidney and brain. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 375298 Human
- Entrez Gene: 228094 Mouse
- Omim: 608381 Human
- SwissProt: Q49MI3 Human
- Unigene: 715753 Human
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别名
- Ceramide kinase like protein antibody
- Ceramide kinase-like protein antibody
- CERKL antibody
see all
图片
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Western blot - Anti-CERKL antibody (ab222828)Anti-CERKL antibody (ab222828) at 1/1000 dilution + Mouse lung tissue lysate
Secondary
Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 20,25,43,48,53,58,60,63 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)Paraffin-embedded human prostate cancer tissue were stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)Paraffin-embedded human pancreatic tissue stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (ab222828)MCF7 (human breast adenocarcinoma cell line) cells stained for CERKL (green) using ab222828 at 1/100 dilution in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab222828 尚未被引用在任何文献中。