Anti-Ceramide glucosyltransferase抗体(ab124296)
Key features and details
- Rabbit polyclonal to Ceramide glucosyltransferase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Ceramide glucosyltransferase抗体 -
描述
兔多克隆抗体to Ceramide glucosyltransferase -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide corresponding to Human Ceramide glucosyltransferase aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab140400) -
阳性对照
- This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124296于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase". -
组织特异性
Found in all tissues examined. -
通路
Lipid metabolism; sphingolipid metabolism. -
序列相似性
Belongs to the glycosyltransferase 2 family. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7357 Human
- Entrez Gene: 22234 Mouse
- Entrez Gene: 83626 Rat
- Omim: 602874 Human
- SwissProt: Q16739 Human
- SwissProt: O88693 Mouse
- SwissProt: Q9R0E0 Rat
- Unigene: 304249 Human
see all -
别名
- CEGT_HUMAN antibody
- Ceramide glucosyltransferase antibody
- GCS antibody
see all
图片
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Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 : Spleen (Rat) Tissue Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa, 36 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124296 被引用在 3 文献中.
- Chueakwon P et al. Inhibition of Ceramide Glycosylation Enhances Cisplatin Sensitivity in Cholangiocarcinoma by Limiting the Activation of the ERK Signaling Pathway. Life (Basel) 12:N/A (2022). PubMed: 35330102
- Hayashi Y et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem 293:17505-17522 (2018). PubMed: 30242129
- Sun R et al. Lowered humidity produces human epidermal equivalents with enhanced barrier properties. Tissue Eng Part C Methods 21:15-22 (2015). PubMed: 24803151