Anti-CEP295抗体(ab122490)
Key features and details
- Rabbit polyclonal to CEP295
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CEP295抗体 -
描述
兔多克隆抗体to CEP295 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CEP295 aa 1877-1978.
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阳性对照
- ICC/IF: U-2 OS cells. WB: Human liver lysate. IHC-P: Human prostate tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122490于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/250 - 1/500.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/250 - 1/500. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 85459 Human
- SwissProt: Q9C0D2 Human
- Unigene: 458418 Human
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别名
- hCG_2036584 antibody
- K1731_HUMAN antibody
- Kiaa1731 antibody
- Protein KIAA1731 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.
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Western blot - Anti-CEP295 antibody (ab122490)All lanes : Anti-CEP295 antibody (ab122490) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab122490 被引用在 6 文献中.
- Atorino ES et al. CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion. Nat Commun 11:903 (2020). PubMed: 32060285
- Kim J et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). PubMed: 30814333
- Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). PubMed: 29752437
- Fong CS et al. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Mol Biol Cell 29:2801-2808 (2018). PubMed: 30230954
- Sydor AM et al. PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN. Elife 7:N/A (2018). PubMed: 30168418
- Fu J et al. Conserved molecular interactions in centriole-to-centrosome conversion. Nat Cell Biol 18:87-99 (2016). PubMed: 26595382