Anti-CEP152抗体(ab183911)
Key features and details
- Rabbit polyclonal to CEP152
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CEP152抗体
参阅全部 CEP152 一抗 -
描述
兔多克隆抗体to CEP152 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human CEP152 aa 58-264. The exact sequence is proprietary.
Database link: O94986 -
阳性对照
- WB: HEK-293T and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 189 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 189 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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疾病相关
Defects in CEP152 are the cause of microcephaly primary type 4 (MCPH4) [MIM:604321]. A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 22995 Human
- Omim: 613529 Human
- SwissProt: O94986 Human
- Unigene: 443005 Human
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别名
- Asterless antibody
- CE152_HUMAN antibody
- Centrosomal protein 152kDa antibody
see all
图片
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Immunofluorescent analysis of HeLa cells (fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min) labeling CEP152 with ab183911 at 1/2000 dilution. Right panel costained with Hoechst 33342 (blue) and alpha 1b tubulin antibody at 1/5000 dilution (red).
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All lanes : Anti-CEP152 antibody (ab183911) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 189 kDa5% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab183911 被引用在 12 文献中.
- Fang CT et al. Inhibition of acetyl-CoA carboxylase impaired tubulin palmitoylation and induced spindle abnormalities. Cell Death Discov 9:4 (2023). PubMed: 36617578
- Chen F et al. Self-assembly of pericentriolar material in interphase cells lacking centrioles. Elife 11:N/A (2022). PubMed: 35787744
- Meitinger F et al. TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. J Cell Biol 220:N/A (2021). PubMed: 33983387
- Meitinger F et al. TRIM37 controls cancer-specific vulnerability to PLK4 inhibition. Nature 585:440-446 (2020). PubMed: 32908304
- Fang CT et al. HSP70 regulates Eg5 distribution within the mitotic spindle and modulates the cytotoxicity of Eg5 inhibitors. Cell Death Dis 11:715 (2020). PubMed: 32873777