AB75005

Anti-CEP135抗体

Name: CEP135抗体 (ab75005)| Abcam中文官网
Brand: Abcam Limited
SKU: AB75005
Price: 4629 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-CEP135 antibody

(1 Review)

(36 Publications)

Rabbit Polyclonal CEP135 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CEP135.

查看别名

CEP4, KIAA0635, CEP135, Centrosomal protein of 135 kDa, Cep135, Centrosomal protein 4

1 Images

Unknown

Western blot - Anti-CEP135 antibody (AB75005)

All lanes:

Western blot - Anti-CEP135 antibody (ab75005) at 1/500 dilution

Lane 1:

extracts from COLO cells at 30 µg

Lane 2:

extracts from COLO cells at 30 µg with immunising peptide

Predicted band size: 133 kDa

Observed band size: 134 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

ELISA, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human CEP135. The exact immunogen used to generate this antibody is proprietary information.

Q66GS9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CEP135 also known as centrosomal protein 135kDa is an important structural component of the centrosome an important cellular organelle responsible for organizing microtubules during cell division. The protein has a molecular mass of approximately 135 kDa. It localizes mainly to the centrosome appearing in various cell types where it assists in the maintenance of centrosome integrity. Its expression is not limited to a single tissue; instead it occurs broadly underlining its importance in cellular processes reliant on centrosome function.

Biological function summary

CEP135 belongs to the percentriolar matrix where it plays a role in centrosome cohesion and spindle formation during mitosis. The protein contributes significantly to the assembly of the centrosomal complex working in concert with other structural proteins. Within this complex it acts to ensure the centrosome can correctly duplicate and segregate essential steps for accurate cell division and maintenance of genomic stability.

Pathways

CEP135 engages in the microtubule nucleation pathway and participates in bipolar spindle assembly both fundamental for cell division. Its function aligns closely with other centrosomal proteins such as γ-tubulin that are critical for the initiation and organization of microtubules. CEP135's activity must align with these pathways to facilitate correct chromosomal alignment and segregation a process essential for avoiding aneuploidy.

CEP135 connects mainly to microcephaly and some forms of cancer. Mutations in the CEP135 gene can lead to microcephaly a condition characterized by reduced brain size due to defective neurogenesis. This situation implicates CEP135's importance in brain development. Additionally CEP135's role in ensuring accurate segregation of chromosomes can influence cancer progression where centrosome defects and chromosomal instability are common. Relations with proteins like pericentrin highlight the broader implications of CEP135's dysfunction in pathological states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Centrosomal microtubule-binding protein involved in centriole biogenesis (PubMed : 27477386). Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (PubMed : 27185865).

See full target information CEP135

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38920633

2024

The Immune Checkpoint Protein PD-L1 Regulates Ciliogenesis and Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ewud Agborbesong,Xiaogang Li

Life science alliance 6: PubMed36384894

2022

GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Bühler,Jeanine Fahrländer,Alexander Sauter,Markus Becker,Elisa Wistorf,Matthias Steinfath,Ailine Stolz

PLoS biology 20:e3001854 PubMed36282799

2022

cNap1 bridges centriole contact sites to maintain centrosome cohesion.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Mahen

The EMBO journal 41:e108739 PubMed35678476

2022

Centrosome function is critical during terminal erythroid differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Péter Tátrai,Fanni Gergely

Cells 11: PubMed35406752

2022

Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human Knockout Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Chu,Oliver J Gruss

EMBO reports 23:e54160 PubMed34957672

2021

Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Magistrati,Giorgia Maestrini,Carlos A Niño,Mariana Lince-Faria,Galina Beznoussenko,Alexandre Mironov,Elena Maspero,Mónica Bettencourt-Dias,Simona Polo

Science advances 7: PubMed34162535

2021

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Chen,Qingwen Xu,Yuxia Zhang,Brian A Davies,Yan Huang,David J Katzmann,Peter C Harris,Jinghua Hu,Kun Ling

eLife 10: PubMed33491649

2021

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre Gönczy

Molecular therapy. Nucleic acids 22:815-831 PubMed33230478

2020

Circular RNA circRIMS1 Acts as a Sponge of miR-433-3p to Promote Bladder Cancer Progression by Regulating CCAR1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Feifan Wang,Mengjing Fan,Yueshu Cai,Xuejian Zhou,Shengcheng Tai,Yanlan Yu,Hongshen Wu,Yan Zhang,Jiaxin Liu,Shihan Huang,Ning He,Zhenghui Hu,Xiaodong Jin

Nature 585:440-446 PubMed32908304

2020

TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Franz Meitinger,Midori Ohta,Kian-Yong Lee,Sadanori Watanabe,Robert L Davis,John V Anzola,Ruth Kabeche,David A Jenkins,Andrew K Shiau,Arshad Desai,Karen Oegema

View all publications

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Anti-CEP135抗体

Anti-CEP135 antibody

Western blot - Anti-CEP135 antibody (AB75005)