Anti-Centrobin抗体(ab70448)
Key features and details
- Mouse polyclonal to Centrobin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Centrobin抗体 -
描述
小鼠多克隆抗体to Centrobin -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Centrobin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH21134.1, 1 a.a. ~ 903 a.a).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70448于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa).
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa). |
靶标
-
功能
Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis. -
组织特异性
Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. -
发展阶段
Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole. - Information by UniProt
-
数据库链接
- Entrez Gene: 116840 Human
- Omim: 611425 Human
- SwissProt: Q8N137 Human
- Unigene: 348012 Human
-
别名
- 9830165K03Rik antibody
- Centrobin antibody
- centrobin, centrosomal BRCA2 interacting protein antibody
see all
图片
-
Western blot - Anti-Centrobin antibody (ab70448)All lanes : Anti-Centrobin antibody (ab70448) at 1/500 dilution
Lane 1 : Centrobin transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
数据表及文件
-
Datasheet download
文献 (26)
ab70448 被引用在 26 文献中.
- Hall EA et al. Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis. Elife 12:N/A (2023). PubMed: 36790165
- Uzbas F & O'Neill AC Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells. Bio Protoc 13:e4812 (2023). PubMed: 37727868
- Moraes de Farias K et al. The Isoflavanoid (+)-PTC Regulates Cell-Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line. Chem Biodivers 19:e202200102 (2022). PubMed: 35362194
- Le Roux-Bourdieu M et al. PLK1 controls centriole distal appendage formation and centrobin removal via independent pathways. J Cell Sci 135:N/A (2022). PubMed: 35343570
- Karasu OR et al. The central scaffold protein CEP350 coordinates centriole length, stability, and maturation. J Cell Biol 221:N/A (2022). PubMed: 36315013
