Anti-Centaurin alpha 1抗体(ab27476)
Key features and details
- Goat polyclonal to Centaurin alpha 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Centaurin alpha 1抗体
参阅全部 Centaurin alpha 1 一抗 -
描述
山羊多克隆抗体to Centaurin alpha 1 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide:
CQEYAVEAHFKHKP
, corresponding to amino acids 362-374 of Human Centaurin alpha 1. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27476于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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相关性
The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP. -
细胞定位
Cytoplasmic, Nuclear and Plasma membrane -
数据库链接
- Entrez Gene: 11033 Human
- Entrez Gene: 231821 Mouse
- Entrez Gene: 397453 Pig
- Entrez Gene: 171097 Rat
- Omim: 608114 Human
- SwissProt: O75689 Human
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别名
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
see all
图片
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Western blot - Anti-Centaurin alpha 1 antibody (ab27476)All lanes : Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Pig brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (ab27476)IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (4)
ab27476 被引用在 4 文献中.
- Gutiérrez Y et al. KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling. J Cell Biol 220:N/A (2021). PubMed: 33999113
- Van Duzer A et al. ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival. Life Sci Alliance 2:N/A (2019). PubMed: 31792062
- Szatmari EM et al. Centaurin-a1-Ras-Elk-1 signaling at mitochondria mediates ß-amyloid-induced synaptic dysfunction. J Neurosci 33:5367-74 (2013). WB ; Rat . PubMed: 23516302
- Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P ; Human, Rat . PubMed: 17635995
