AB27476

Anti-Centaurin alpha 1抗体

Anti-Centaurin alpha 1 antibody

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(4 Publications)

Goat Polyclonal Centaurin alpha 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADAP1 aa 350-400.

查看别名

CENTA1, ADAP1, Arf-GAP with dual PH domain-containing protein 1, Centaurin-alpha-1, Putative MAPK-activating protein PM25, Cnt-a1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-Centaurin alpha 1 antibody (AB27476)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/mL

Lane 1:

Human brain tissue lysate at 35 µg

Lane 2:

Mouse brain tissue lysate at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

Lane 4:

Pig brain tissue lysate at 35 µg

Predicted band size: 42 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human, Pig

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human ADAP1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

O75689

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Regulates the incorporation of CD63 and CD9 into multivesicular bodies (PubMed : 38682696).

See full target information ADAP1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 220: PubMed33999113

2021

KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Yolanda Gutiérrez,Sergio López-García,Argentina Lario,Silvia Gutiérrez-Eisman,Cédric Delevoye,José A Esteban

Life science alliance 2: PubMed31792062

2019

ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Avery Van Duzer,Sachiko Taniguchi,Ajit Elhance,Takahiro Tsujikawa,Naoki Oshimori

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:5367-74 PubMed23516302

2013

Centaurin-α1-Ras-Elk-1 signaling at mitochondria mediates β-amyloid-induced synaptic dysfunction.

Applications

Species

Rat

Erzsebet M Szatmari,Ana F Oliveira,Elizabeth J Sumner,Ryohei Yasuda

Journal of cell science 120:2683-93 PubMed17635995

2007

The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.

Applications

IHC-P, ICC/IF, IHC-P, WB, ICC/IF, WB

Species

Rat, Human, Human, Human, Rat, Rat

Carlene D Moore,Erin E Thacker,Jennifer Larimore,David Gaston,Alison Underwood,Brian Kearns,Sean I Patterson,Trevor Jackson,Chris Chapleau,Lucas Pozzo-Miller,Anne Theibert

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Centaurin alpha 1抗体

Anti-Centaurin alpha 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

Western blot - Anti-Centaurin alpha 1 antibody (AB27476)