Anti-CENPI抗体(ab118796)
Key features and details
- Rabbit polyclonal to CENPI
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CENPI抗体
参阅全部 CENPI 一抗 -
描述
兔多克隆抗体to CENPI -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human CENPI aa 50-150.
Database link: Q92674 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 87 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 87 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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相关性
CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 2491 Human
- Omim: 300065 Human
- SwissProt: Q92674 Human
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别名
- CENP I antibody
- centromere protein I antibody
- Follicle simulating hormone primary response protein antibody
see all
图片
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Western blot - Anti-CENPI antibody (ab118796)All lanes : Anti-CENPI antibody (ab118796) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-CENPI antibody (ab118796)Detection of CENPI in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118796 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab118796 被引用在 4 文献中.
- Wang J et al. Centromere Protein I (CENP-I) Is Upregulated in Gastric Cancer, Predicts Poor Prognosis, and Promotes Tumor Cell Proliferation and Migration. Technol Cancer Res Treat 20:15330338211045510 (2021). PubMed: 34617858
- Engqvist H et al. Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry. Front Oncol 10:162 (2020). PubMed: 32133296
- Kyriacou E & Heun P High-resolution mapping of centromeric protein association using APEX-chromatin fibers. Epigenetics Chromatin 11:68 (2018). PubMed: 30445992
- Thangavelu PU et al. Overexpression of the E2F target geneCENPIpromotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer. Oncotarget 8:62167-62182 (2017). PubMed: 28977935