Anti-CENPA (phospho S7)抗体(ab62242)
Key features and details
- Rabbit polyclonal to CENPA (phospho S7)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CENPA (phospho S7)抗体
参阅全部 CENPA 一抗 -
描述
兔多克隆抗体to CENPA (phospho S7) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CENPA aa 1-100 (phospho S7).
Database link: P49450 -
阳性对照
- HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62242 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/500 - 1/1000.
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| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
序列相似性
Belongs to the histone H3 family. -
结构域
The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres. -
翻译后修饰
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height. - Information by UniProt
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数据库链接
- Entrez Gene: 1058 Human
- Omim: 117139 Human
- SwissProt: P49450 Human
- Unigene: 1594 Human
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别名
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (ab62242)This image is courtesy of an abreview by Kirk Mcmanus
ab62242 staining CENPA (phospho S7) in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. Undiluted ab150081 was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (ab62242)ab62242 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62242 被引用在 1 文献中.
- Santaguida S et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol 190:73-87 (2010). WB, ICC/IF ; Human . PubMed: 20624901