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Anti-CENH3抗体(ab72001)

Submit a review Q&A (2)References (6)

Key features and details

  • Rabbit polyclonal to CENH3
  • Suitable for: ICC, WB
  • Reacts with: Arabidopsis thaliana
  • Isotype: IgG

概述

  • 产品名称

    Anti-CENH3抗体

  • 描述

    兔多克隆抗体to CENH3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC, WBmore details

  • 种属反应性

    与反应: Arabidopsis thaliana

  • 免疫原

    Synthetic peptide corresponding to Arabidopsis thaliana CENH3 (N terminal).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Constituent: Whole serum
  • Concentration information loading...

  • 纯度

    Whole antiserum

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab72001于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 相关性

    HTR12 is a histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation and may serve as an epigenetic mark that propagates centromere identity through replication and cell division.

  • 细胞定位

    Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.

  • 数据库链接

    • 别名

      • CENH3 antibody
      • Centromeric histone CENH3 antibody
      • F6F3.17 antibody
      • F6F3_17 antibody
      • Histone H3 like centromeric protein HTR12 antibody
      • HTR 12 antibody
      • HTR12 antibody
      see all

    文献 (6)

    发表研究结果有使用 ab72001？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab72001 被引用在 6 文献中.

    • Capitao C  et al. A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. PLoS Genet 17:e1009779 (2021). PubMed: 34591845
    • Naish M  et al. The genetic and epigenetic landscape of the Arabidopsis centromeres. Science 374:eabi7489 (2021). PubMed: 34762468
    • Xu RY  et al. The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis. New Phytol 224:229-241 (2019). PubMed: 31230348
    • Antosch M  et al. Mitotic lifecycle of chromosomal 3xHMG-box proteins and the role of their N-terminal domain in the association with rDNA loci and proteolysis. New Phytol 208:1067-77 (2015). PubMed: 26213803
    • Karimi-Ashtiyani R  et al. Point mutation impairs centromeric CENH3 loading and induces haploid plants. Proc Natl Acad Sci U S A 112:11211-6 (2015). WB . PubMed: 26294252
    • Mérai Z  et al. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proc Natl Acad Sci U S A 111:16166-71 (2014). PubMed: 25344531

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question


    I would like to know what epitope was used for its production and if possible any references and its specificity towards CENH3 compared to other histones.

    Read More
    Answer


    Unfortunately the immunogen sequence is considered proprietary (commercially sensitive and confidential) and I can not disclose it at this time. However, I can confirm that the immunogen is a peptide of 20 amino acids located between 1 and 40 amino acids of the A. thaliana HTR12.

    There are currently no references for ab72001. Regarding cross reactivity, the lab have confirmed that ab72001 shows no cross-reactivity with other histones.

    Read More

    Question

    I am interested in ab72001 product, but I need the information regarding immunogen sequence used to make the antibody, to design my experiment in a better way..
    and expecting your reply soon
    thanks

    Read More
    Answer

    Thank you very much for contacting us with your question.

    Unfortunately the immunogen sequence is considered commercially sensitive and we can not disclose it at this time. I can onlyconfirm that it is taken from the first 100 N-terminal amino acids.

    If you'd like to describe the experiment that you would like to design, I may be able to help you further. Could you tell me what kind of assay you're planningto run and what kind of samples you will use?

    I look forward to hearing from you.I am sorry thatI do not have the information that you're looking for, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

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