Anti-Cenexin1/ODF2抗体(ab43840)
Key features and details
- Rabbit polyclonal to Cenexin1/ODF2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cenexin1/ODF2抗体
参阅全部 Cenexin1/ODF2 一抗 -
描述
兔多克隆抗体to Cenexin1/ODF2 -
宿主
Rabbit -
特异性
On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa). -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Cenexin1/ODF2 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab43839) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab43840于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml.
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ICC/IF | (3) |
1/400.
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说明 |
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WB
Use a concentration of 1 µg/ml. |
ICC/IF
1/400. |
靶标
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功能
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly. -
组织特异性
Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails. -
序列相似性
Belongs to the ODF2 family. -
翻译后修饰
Tyrosine phosphorylated. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles. - Information by UniProt
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数据库链接
- Entrez Gene: 4957 Human
- Entrez Gene: 18286 Mouse
- Omim: 602015 Human
- SwissProt: Q5BJF6 Human
- SwissProt: A3KGV1 Mouse
- Unigene: 129055 Human
- Unigene: 330116 Mouse
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别名
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
- Cenexin 1 antibody
see all
图片
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All lanes : ab43840 at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg
Lane 2 : MCF7 (Human breast adenocarcinoma) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840)This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.
Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.
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All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution
Lane 1 : 25 ug HeLa cell lysate
Lane 2 : 12.5 ug HeLa cell lysate
Lane 3 : 25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Lane 4 : 12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Secondary
All lanes : Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: ~75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab43840 被引用在 36 文献中.
- Uzbas F & O'Neill AC Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells. Bio Protoc 13:e4812 (2023). PubMed: 37727868
- Ali A et al. Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG. Nat Commun 14:289 (2023). PubMed: 36702836
- Zhu ZJ et al. Novel mutation in ODF2 causes multiple morphological abnormalities of the sperm flagella in an infertile male. Asian J Androl 24:463-472 (2022). PubMed: 35102900
- Magistrati E et al. Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1. EMBO Rep 23:e54160 (2022). PubMed: 34957672
- Klebanovych A et al. C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress. Cells 11:N/A (2022). PubMed: 35159364