Anti-Cenexin1/ODF2抗体(ab125192)
Key features and details
- Rabbit polyclonal to Cenexin1/ODF2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cenexin1/ODF2抗体
参阅全部 Cenexin1/ODF2 一抗 -
描述
兔多克隆抗体to Cenexin1/ODF2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Bat
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免疫原
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human Cenexin1/ODF2 (NP_702911.1).
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阳性对照
- HeLa whole cell lysate 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. The product can be stored for up to 12 months. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125192于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 95 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly. -
组织特异性
Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails. -
序列相似性
Belongs to the ODF2 family. -
翻译后修饰
Tyrosine phosphorylated. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles. - Information by UniProt
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数据库链接
- Entrez Gene: 491315 Dog
- Entrez Gene: 4957 Human
- Entrez Gene: 18286 Mouse
- Entrez Gene: 100156735 Pig
- Entrez Gene: 29479 Rat
- Omim: 602015 Human
- SwissProt: Q5BJF6 Human
- SwissProt: A3KGV1 Mouse
see all -
别名
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
- Cenexin 1 antibody
see all
图片
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Western blot - Anti-Cenexin1/ODF2 antibody (ab125192)All lanes : Anti-Cenexin1/ODF2 antibody (ab125192) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Cenexin1/ODF2 antibody (ab125192)Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab125192 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in laneWestern Blot: Cenexin1/ODF2 antibody (ab125192) at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125192 尚未被引用在任何文献中。