AB250874

重组Anti-Cenexin1/ODF2抗体[EPR13561] - BSA and Azide free

Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Cenexin1/ODF2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

Outer dense fiber protein 2, Cenexin, Outer dense fiber of sperm tails protein 2, ODF2

3 Images

Flow Cytometry (Intracellular) - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of paraformaldehyde fixed Hela cells labeling Cenexin1/ODF2 with ab186412 at 1/250 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution

Supplier Data

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] (<a href='/products/primary-antibodies/cenexin1-odf2-antibody-epr13561-ab186412'>ab186412</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 95 kDa

false

Supplier Data

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] (<a href='/products/primary-antibodies/cenexin1-odf2-antibody-epr13561-ab186412'>ab186412</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 95 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-Cenexin1/ODF2 antibody [EPR13561]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13561

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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产品详情

ab250874 is the carrier-free version of ab186412.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

See full target information Outer dense fiber protein 2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-Cenexin1/ODF2抗体[EPR13561] - BSA and Azide free