AB43840

Anti-Cenexin1/ODF2抗体

Name: Cenexin1/ODF2抗体 (ab43840)| Abcam中文官网
Brand: Abcam Limited
SKU: AB43840
Price: 3719 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-Cenexin1/ODF2 antibody

(4 Reviews)

(40 Publications)

Rabbit Polyclonal Cenexin1/ODF2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 40 publications.

查看别名

Outer dense fiber protein 2, Cenexin, Outer dense fiber of sperm tails protein 2, ODF2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (AB43840)

ICC/IF

AbReview58215****

Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (AB43840)

Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

Lab

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

ab43840 at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg

Lane 2:

MCF7 (Human breast adenocarcinoma) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 95 kDa

Observed band size: 100 kDa

true

Exposure time: 20min

AbReview17722****

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution

Lane 1:

25 ug HeLa cell lysate

Lane 2:

12.5 ug HeLa cell lysate

Lane 3:

25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (<a href='/products/unavailable/human-cenexin1odf2-peptide-ab43839'>ab43839</a>)

Lane 4:

12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (<a href='/products/unavailable/human-cenexin1odf2-peptide-ab43839'>ab43839</a>)

Secondary

All lanes:

Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution

Predicted band size: 95 kDa

Observed band size: ~75 kDa

true

Exposure time: 4min

Image courtesy of an anonymous abreview.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa).

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

See full target information Outer dense fiber protein 2

style

left-column

文献 (40)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40408364

2025

Aldehyde metabolism governs resilience of mucociliary clearance to air pollution exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Shinjyo,Haruna Kimura,Tomomi Yoshihara,Jun Suzuki,Masaya Yamaguchi,Shigetada Kawabata,Yasutaka Okabe

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308820 PubMed38634253

2024

The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Huicheng Chen,Zhimao Wu,Ziwei Yan,Chuan Chen,Yingying Zhang,Qiaoling Wang,Yuqing Gao,Kun Ling,Jinghua Hu,Qing Wei

Bio-protocol 13:e4812 PubMed37727868

2023

Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Uzbas,Adam C O'Neill

Nature communications 14:289 PubMed36702836

2023

Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.

Applications

Unspecified application

Species

Unspecified reactive species

Aamir Ali,Chithran Vineethakumari,Cristina Lacasa,Jens Lüders

iScience 25:104476 PubMed35721463

2022

Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Fdez,Jesús Madero-Pérez,Antonio J Lara Ordóñez,Yahaira Naaldijk,Rachel Fasiczka,Ana Aiastui,Javier Ruiz-Martínez,Adolfo López de Munain,Sally A Cowley,Richard Wade-Martins,Sabine Hilfiker

Development (Cambridge, England) 149: PubMed35420656

2022

Planar cell polarity-dependent asymmetric organization of microtubules for polarized positioning of the basal body in node cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorei Sai,Yayoi Ikawa,Hiromi Nishimura,Katsutoshi Mizuno,Eriko Kajikawa,Takanobu A Katoh,Toshiya Kimura,Hidetaka Shiratori,Katsuyoshi Takaoka,Hiroshi Hamada,Katsura Minegishi

Journal of cell science 135: PubMed35343570

2022

PLK1 controls centriole distal appendage formation and centrobin removal via independent pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan Le Roux-Bourdieu,Devashish Dwivedi,Daniela Harry,Patrick Meraldi

Cells 11: PubMed35159364

2022

C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiya Klebanovych,Stanislav Vinopal,Eduarda Dráberová,Vladimíra Sládková,Tetyana Sulimenko,Vadym Sulimenko,Věra Vosecká,Libor Macůrek,Agustin Legido,Pavel Dráber

Asian journal of andrology 24:463-472 PubMed35102900

2022

Novel mutation in causes multiple morphological abnormalities of the sperm flagella in an infertile male.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Jue Zhu,Yi-Zhou Wang,Xiao-Bo Wang,Chen-Cheng Yao,Liang-Yu Zhao,Zhen-Bo Zhang,Yu Wu,Wei Chen,Zheng Li

EMBO reports 23:e54160 PubMed34957672

2021

Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Magistrati,Giorgia Maestrini,Carlos A Niño,Mariana Lince-Faria,Galina Beznoussenko,Alexandre Mironov,Elena Maspero,Mónica Bettencourt-Dias,Simona Polo

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cenexin1/ODF2抗体

Anti-Cenexin1/ODF2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (AB43840)

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性