重组Anti-CEND1抗体[EPR3739] (ab113076)
![Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)](https://www.abcam.cn/ps/products/113/ab113076/Images/ab113076-125805-anti-cend1-antibody-epr3739-western-blot.jpg "Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3739] to CEND1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CEND1抗体[EPR3739]
参阅全部 CEND1 一抗 -
描述
兔单克隆抗体[EPR3739] to CEND1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal brain lysate, U-87 MG and Neuro 2a cell lysates; Human brain glioma tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3739 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113076于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min |
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa). |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min |
靶标
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相关性
CEND1 is a neuron-specific protein. The similar protein in pig enhances neuroblastoma cell differentiation in vitro and may be involved in neuronal differentiation in vivo. Multiple pseudogenes have been reported for this gene. -
细胞定位
Membrane; Single-pass type IV membrane protein. -
数据库链接
- Entrez Gene: 51286 Human
- Entrez Gene: 57754 Mouse
- Entrez Gene: 361675 Rat
- Omim: 608213 Human
- SwissProt: Q8N111 Human
- SwissProt: Q9JKC6 Mouse
- SwissProt: Q5FVI4 Rat
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别名
- BM88 antibody
- BM88 antigen antibody
- Cell cycle exit and neuronal differentiation 1 antibody
see all
图片
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Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)All lanes : Anti-CEND1 antibody [EPR3739] (ab113076) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)](https://www.abcam.cn/ps/products/113/ab113076/Images/ab113076-125804-anti-cend1-antibody-epr3739-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)](https://www.abcam.cn/ps/products/113/ab113076/Images/ab113076-125803-anti-cend1-antibody-epr3739-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human glioma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-CEND1 antibody [EPR3739] (ab113076)](https://www.abcam.cn/ps/products/113/ab113076/Images/ab113076-5-benefits-of-recombinant-antibodies.png)
Anti-CEND1 antibody [EPR3739] (ab113076)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab113076 被引用在 3 文献中.
- Wang R et al. Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury. Neural Regen Res 17:130-136 (2022). PubMed: 34100448
- Zhang L et al. Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation. Cells 9:N/A (2020). PubMed: 32630355
- Scaturro P et al. An orthogonal proteomic survey uncovers novel Zika virus host factors. Nature 561:253-257 (2018). PubMed: 30177828