AB247541

重组Anti-CEND1抗体[EPR3738] - BSA and Azide free

Anti-CEND1 antibody [EPR3738] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CEND1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

BM88, CEND1, Cell cycle exit and neuronal differentiation protein 1, BM88 antigen

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3738] - BSA and Azide free (AB247541)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3738] - BSA and Azide free (AB247541)

This data was developed using ab92318, the same antibody clone in a different buffer formulation.

ab92318, at a 1/100 dilution, staining CEND1 in paraffin-embedded Human brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CEND1 antibody [EPR3738] - BSA and Azide free (AB247541)

This data was developed using ab92318, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CEND1 antibody [EPR3738] (<a href='/products/primary-antibodies/cend1-antibody-epr3738-ab92318'>ab92318</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-CEND1 antibody [EPR3738]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3738

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab247541 is the carrier-free version of ab92318.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CEND1 also known as cell cycle exit and neuronal differentiation 1 is a protein with a mass of approximately 16 kDa. Researchers have identified its expression specifically in neural precursor cells of the central nervous system. CEND1 plays a role in controlling neuronal differentiation by interacting with cell cycle regulators. This function highlights its importance in the development of the nervous system and its involvement in maintaining the balance between proliferation and differentiation in neurons.

Biological function summary

CEND1 facilitates neuronal outgrowth and maturation without forming part of a larger multiprotein complex. It acts directly on governing neuronal progenitor cell fate promoting differentiation over proliferation. Researchers have found CEND1 to affect cell cycle arrest in neuronal populations steering progenitor cells towards post-mitotic states essential for healthy neural tissue maturation. The presence of CEND1 in neurogenesis stages establishes it as an important player in the shaping of the neuronal landscape in the brain.

Pathways

Several cell cycle-related pathways involve CEND1 in regulating the transition from proliferation to differentiation in neural progenitors. It interacts with key proteins such as p27^Kip1 and cyclin-dependent kinases. These interactions are important in coordinating neurogenesis and neuronal pathway specification. CEND1 ensures proper function and cooperation with other elements of the neurogenic program including pathways like Notch signaling to regulate the temporal and spatial aspects of neuronal differentiation.

Research has linked CEND1 to neurodevelopmental conditions such as microcephaly and certain forms of epilepsy. Malfunctions or dysregulation of CEND1 can result in impaired neuronal differentiation leading to disturbed neural circuit formation. In these contexts CEND1 interacts with proteins like p53 which governs cell cycle arrest and apoptosis offering insights into how disrupted CEND1 expression contributes to the pathophysiology of these neurological disorders. Understanding these relationships provides potential therapeutic targets for correcting or mitigating such conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in neuronal differentiation.

See full target information CEND1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He

View all publications

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-CEND1抗体[EPR3738] - BSA and Azide free