Anti-Cellular Apoptosis Susceptibility/CSE1L抗体(ab70547)

False colour image of Western blot: Anti-Cellular Apoptosis Susceptibility/CSE1L antibody staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab70547 was shown to bind specifically to Cellular Apoptosis Susceptibility/CSE1L. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 30 seconds exposure time. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution.

Paraffin embedded mouse plasmacytoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.

Paraffin embedded human testicular seminoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.

Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.

Affinity purified rabbit anti-CSE1 antibody used for IP at 6 µg per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-CSE1 antibody BL1977.

For blotting immunoprecipitated CSE1, ab70547 was used at 1 µg/ml. Chemiluminescence had an exposure time of 10 seconds.