Anti-Cellubrevin抗体(ab43080)
Key features and details
- Rabbit polyclonal to Cellubrevin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-Cellubrevin抗体
参阅全部 Cellubrevin 一抗 -
描述
兔多克隆抗体to Cellubrevin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Rat Cellubrevin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab43579) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab43080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 22319 Mouse
- Entrez Gene: 29528 Rat
- SwissProt: P63024 Mouse
- SwissProt: P63025 Rat
- Unigene: 273930 Mouse
- Unigene: 219999 Rat
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别名
- CEB antibody
- Cellubrevin antibody
- Synaptobrevin 3 antibody
see all
图片
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Western blot - Anti-Cellubrevin antibody (ab43080)All lanes : Anti-Cellubrevin antibody (ab43080) at 1 µg/ml
Lane 1 : Testis (Mouse) Mouse Tissue Lysate
Lane 2 : Brain (Rat) Rat Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab43080)ICC/IF image of ab43080 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellubrevin antibody (ab43080)IHC image of Cellubrevin staining in Mouse normal testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab43080 被引用在 6 文献中.
- Barbet G et al. TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming. Nat Immunol 22:497-509 (2021). PubMed: 33790474
- Vashi N et al. Ca2+-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages. J Biol Chem 292:5144-5165 (2017). PubMed: 28174296
- Ligeon LA et al. Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles. Autophagy 10:1588-602 (2014). PubMed: 25046114
- Garcia EA et al. Characterization of SNARE proteins in human pituitary adenomas: targeted secretion inhibitors as a new strategy for the treatment of acromegaly? J Clin Endocrinol Metab 98:E1918-26 (2013). PubMed: 24152687
- Schwenk RW et al. Overexpression of Vesicle-associated Membrane Protein (VAMP) 3, but Not VAMP2, Protects Glucose Transporter (GLUT) 4 Protein Translocation in an in Vitro Model of Cardiac Insulin Resistance. J Biol Chem 287:37530-9 (2012). WB, IP ; Rat . PubMed: 22936810
- Mendez M et al. Vesicle-associated Membrane Protein-2 (VAMP2) Mediates cAMP-stimulated Renin Release in Mouse Juxtaglomerular Cells. J Biol Chem 286:28608-18 (2011). WB ; Mouse . PubMed: 21708949