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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-Cellubrevin抗体(ab2102)

  • Datasheet
Reviews (3)Q&A (5)References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab2102)

    Key features and details

    • Rabbit polyclonal to Cellubrevin
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Cellubrevin抗体
      参阅全部 Cellubrevin 一抗
    • 描述

      兔多克隆抗体to Cellubrevin
    • 宿主

      Rabbit
    • 特异性

      Reacts specifically with 10kDa protein of rat CP12 cell extract.
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Mouse
      不与反应: Dog, Human
    • 免疫原

      Synthetic peptide corresponding to Rat Cellubrevin. 15 residue N-terminal synthetic peptide
      Database link: P63025

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: Whole serum
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Cellubrevin protein (ab86097)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2102于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF (1)
    Use at an assay dependent concentration.
    说明
    ICC/IF
    Use at an assay dependent concentration.

    靶标

    • 功能

      SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
    • 序列相似性

      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Membrane. Cell junction > synapse > synaptosome.
    • Target information above from: UniProt accession Q15836 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 22319 Mouse
      • SwissProt: P63024 Mouse
      • Unigene: 273930 Mouse
      • 别名

        • CEB antibody
        • Cellubrevin antibody
        • Synaptobrevin 3 antibody
        • Synaptobrevin-3 antibody
        • VAMP 3 antibody
        • VAMP-3 antibody
        • VAMP3 antibody
        • VAMP3_HUMAN antibody
        • Vesicle associated membrane protein 3 antibody
        • Vesicle-associated membrane protein 3 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab2102)
        Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab2102)

        This picture was kindly supplied as part of the review submitted by Jennifer Chua . The image shows staining in the perinuclear region and in the peripheral endosomal structures of RAW 264.7 cells stained with ab2102 (at a dilution of 1/50 with a secondary antibody conjugated with Alexa 568).

      实验方案

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab2102?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2102 被引用在 2 文献中.

      • Imai A  et al. Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane. Arch Oral Biol 48:597-604 (2003). PubMed: 12828989
      • Yamamoto M  et al. Reversible suppression of glutamatergic neurotransmission of cerebellar granule cells in vivo by genetically manipulated expression of tetanus neurotoxin light chain. J Neurosci 23:6759-67 (2003). PubMed: 12890769

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-8 of 8 Abreviews or Q&A

      Western blot abreview for Anti-Cellubrevin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (RBL-2H3 cell)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      RBL-2H3 cell
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 08 2015

      Western blot abreview for Anti-Cellubrevin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (J774 macrophage)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      J774 macrophage
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 15 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cellubrevin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 0.5% · Temperature: 25°C
      Sample
      Mouse Cell (J774 macrophage)
      Specification
      J774 macrophage
      Permeabilization
      Yes - 0.05% saponin
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 28 2014

      Question

      I received 125 uL instead of 200 uL. The order number is 87602 lot number 66469.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 23 2005

      Answer

      Dear Carmen, I am sorry that you received this shortage. I am sending you a free of charge replacement for this item. I apologize for the inconvenience. Best regards, Bill Bill Campbell, Ph.D. Technical Specialist Abcam, Inc. One Kendall Square, Bldg 200 Cambridge, MA 02139 Phone 617-225-2272 (ext. 224) Fax 617-507-5831 www.abcam.com

      Read More

      Abcam Scientific Support

      回复于 Jun 28 2005

      Question

      Ab5789 is listed as a VAMP3 antibody, and ab2102 and ab2103 are listed as Cellubrevin antibodies. Are they the same? Are the peptide sequences available for ab2102 and ab2103? Have any of these antibodies been tested for cross-reactivity with guinea pig?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 22 2005

      Answer

      Regarding ab2102 and ab2103, unfortunately the peptide sequences are not available. If you have any additional questions, please let us know.

      Read More

      Abcam Scientific Support

      回复于 Feb 23 2005

      Question

      Ab5789 is listed as a VAMP3 antibody, and ab2102 and ab2103 are listed as Cellubrevin antibodies. Are they the same? Are the peptide sequences available for ab2102 and ab2103? Have any of these antibodies been tested for cross-reactivity with guinea pig?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 22 2005

      Answer

      Thank you for your enquiry. Cellubrevin is an alternative name for VAMP3, and so these 3 antibodies target the same protein. Ab2103 has been tested for cross-reactivity with Human and Mouse, and does not cross-react with Rat or Dog. Ab2102 cross-reacts with Rat and does not cross-react with Human or Dog. Ab5789 cross-reacts with Dog, Human, Mouse and Rat. So none have been tested yet for cross-reactivity with guinea pig, to our knowledge. I will try to obtain the peptide sequences for ab2102 and ab2103 and will let you know.

      Read More

      Abcam Scientific Support

      回复于 Feb 22 2005

      Question

      Will 2102 recognize cellubrevin in CHO cells for immunofuoresence

      Read More

      Abcam community

      Verified customer

      Asked on Mar 03 2001

      Answer

      This antibody works in immunofluorescence, the suggested working dilution is 1/50. It reacts with rat cellubrevin; rat PC12 cell extract is positive in Western blotting. It should certainly work for IF with CHO cells transfected with rat cellubrevin. (For reactivity with human cellubrevin, use ab2103).

      Read More

      Abcam Scientific Support

      回复于 Mar 05 2001

      Question

      Will this antibody recognize mouse cellubrevin when used in a western blot?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2001

      Answer

      The antibody has not been tested for cross-reactivity with mouse. However, the antibody was raised against a synthetic peptide from the rat N-terminal region, and is thought likely to recognise mouse.

      Read More

      Abcam Scientific Support

      回复于 Jan 29 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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