Anti-CELF3抗体(ab121249)
Key features and details
- Rabbit polyclonal to CELF3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CELF3抗体 -
描述
兔多克隆抗体to CELF3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CELF3 aa 268-401.
Database link: Q5SZQ8 -
阳性对照
- IHC-P: Human cerebral cortex tissue; ICC/IF: SH-SY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
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| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
|
靶标
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功能
RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. -
组织特异性
Expressed in brain. -
序列相似性
Belongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 11189 Human
- Omim: 612678 Human
- SwissProt: Q5SZQ8 Human
- Unigene: 26047 Human
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别名
- Bruno-like protein 1 antibody
- BRUNOL1 antibody
- CAG repeat protein 4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF3 antibody (ab121249)Immunohistochemical staining of paraffin embedded Human cerebral cortex, using 1/35 ab121249, shows distinct cytoplasmic positivity in neurons.
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Immunocytochemistry/ Immunofluorescence - Anti-CELF3 antibody (ab121249)Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate labeling CELF3 with ab121249 at 2 µg/mL.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121249 尚未被引用在任何文献中。