Anti-CEBP Beta抗体[18F8] (ab15049)
![Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.cn/ps/products/15/ab15049/Images/ab15049-22280-anti-cebp-beta-antibody-18f8-western-blot.jpg "Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)"){kind=small}
Key features and details
- Mouse monoclonal [18F8] to CEBP Beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CEBP Beta抗体[18F8]
参阅全部 CEBP Beta 一抗 -
描述
小鼠单克隆抗体[18F8] to CEBP Beta -
宿主
Mouse -
特异性
Ab15049 only detects the LAP* isoform of CEBPG beta. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:MHRLLAWDAACLPPPPAAF
(Peptide available asab39810) -
阳性对照
- Transfected 293T cells, P388D1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
18F8 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15049于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
组织特异性
Expressed at low levels in the lung, kidney and spleen. -
序列相似性
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
结构域
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻译后修饰
Sumoylated by polymeric chains of SUMO2 or SUMO3. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 12608 Mouse
- SwissProt: P28033 Mouse
- Unigene: 439656 Mouse
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别名
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
图片
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Western blot - Anti-CEBP Beta antibody [18F8] (ab15049)
Western blot of the CEBP Beta using ab15049 on transfected CEBP beta.
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![Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)](https://www.abcam.cn/ps/products/15/ab15049/Images/ab15049-22279-anti-cebp-beta-antibody-18f8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CEBP Beta antibody [18F8] (ab15049)Immunofluorescence staining of CEBP beta in P388D1 cells using ab15049.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab15049 被引用在 8 文献中.
- Fafián-Labora JA et al. Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage. Cell Metab 32:71-86.e5 (2020). PubMed: 32574561
- Liu SP et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31020769
- Romanel A et al. Inherited determinants of early recurrent somatic mutations in prostate cancer. Nat Commun 8:48 (2017). PubMed: 28663546
- Jang MA et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). PubMed: 28114367
- Liu Q et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). PubMed: 24912680
- Atwood AA et al. Negative Regulation of C/EBPbeta1 by Sumoylation in Breast Cancer Cells. PLoS One 6:e25205 (2011). WB ; Human . PubMed: 21980398
- Atwood AA & Sealy L Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence. Oncogene 29:6004-15 (2010). WB ; Human . PubMed: 20818427
- Su WC et al. Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene. J Biol Chem 278:51150-8 (2003). PubMed: 14530280