CEBP Alpha/CEBPA重组抗体[EPR27924-90]_CEBP Alpha/CEBPA抗体(ab317442)

产品名称

Anti-CEBP Alpha/CEBPA抗体[EPR27924-90]
参阅全部 CEBP Alpha/CEBPA 一抗
描述

兔单克隆抗体[EPR27924-90] to CEBP Alpha/CEBPA
宿主

Rabbit
经测试应用

适用于: IHC-P, Flow Cyt (Intra), WB, IP, ChIP-sequencingmore details
不适用于: ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: THP-1, THP-1 transfected with siRNA specifically targeting CEBP Alpha/CEBPA, THP-1, RAW 264.7, NR8383, MEF, Human lung, Human liver cancer, Human tonsil, Human spleen, Mouse liver and Rat liver lysates. IHC-P: Human tonsil, Mouse liver, Mouse spleen, Rat liver and Rat spleen tissues. Flow Cyt (Intra): THP-1 cell. IP: THP-1 and MEF whole cell lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR27924-90
同种型

IgG
研究领域

Alternative Versions
- Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (ab317443)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Abpromise™承诺保证使用ab317442于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)		1/5000.
WB		1/1000.
IP		1/30.
ChIP-sequencing		Use 8µg for 10⁷ cells.

说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra) 1/5000.
WB 1/1000.
IP 1/30.
ChIP-sequencing Use 8µg for 10⁷ cells.

应用说明

Is unsuitable for ICC/IF.

功能

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
序列相似性

Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
细胞定位

Nucleus.
Target information above from: UniProt accession P49715 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
- SwissProt: P05554 Rat
- Unigene: 76171 Human
- Unigene: 349667 Mouse
- Unigene: 204833 Rat
see all
别名
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
- C/ebpalpha antibody
- CAP4 antibody
- Caspase 8 precursor antibody
- CBF-A antibody
- CCAAT Enhancer Binding Protein alpha antibody
- CCAAT/enhancer binding protein (C/EBP), alpha antibody
- CCAAT/enhancer-binding protein alpha antibody
- CEBP antibody
- CEBP A antibody
- CEBP alpha antibody
- Cebpa antibody
- CEBPA_HUMAN antibody
- FADD homologous ICE/CED 3 like protease antibody
- FADD like ICE antibody
- FLICE antibody
- ICE like apoptotic protease 5 antibody
- ICE8 antibody
- MACH antibody
- MCH5 antibody
- MORT1 associated CED 3 homolog antibody
see all

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

All lanes : Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442) at 1/1000 dilution

Lane 1 : THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate
Lane 2 : THP-1 transfected with siRNA specifically targeting CEBP Alpha/CEBPA whole cell lysate
Lane 3 : THP-1 whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6 : NR8383 (rat alveolar macrophage) whole cell lysate
Lane 7 : MEF (mouse embryo fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 26, 36, 41 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: Jurkat.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8415748).

The lanes 5-6 were developed using a high sensitivity ECL substrate.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-4: 26 seconds, lane 5: 37 seconds, lane 6: 180 seconds, lane 7: 48 seconds
Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

All lanes : Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442) at 1/1000 dilution

Lane 1 : Human lung tissue lysate
Lane 2 : Human liver cancer tissue lysate
Lane 3 : Human tonsil tissue lysate
Lane 4 : Human spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Developed using the ECL technique.

Observed band size: 41 kDa why is the actual band size different from the predicted?

Exposure time: 70 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

All lanes : Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 41 kDa why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8415748).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver (PMID: 2792758). The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat liver. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling CEBP Alpha/CEBPA with ab317442 at 1/5000 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: Jurkat.
Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

CEBP Alpha/CEBPA was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab317442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2: ab317442 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317442 in THP-1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

CEBP Alpha/CEBPA was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab317442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MEF (mouse embryo fibroblast) whole cell lysate
Lane 2: ab317442 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317442 in MEF whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.
ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.
ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.
Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

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