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重组RabMAb

重组Anti-CEBP Alpha/CEBPA抗体[EP709Y] (ab40764)

Reviews (3) Submit a question References (51)
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
  • Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CEBP Alpha/CEBPA抗体[EP709Y]
    参阅全部 CEBP Alpha/CEBPA 一抗

  • 描述

    兔单克隆抗体[EP709Y] to CEBP Alpha/CEBPA

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • U937 cell lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40764于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
说明
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

  • 功能

    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

  • 序列相似性

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch 5 antibody
    • C/EBP alpha antibody
    • C/ebpalpha antibody
    • CAP4 antibody
    • Caspase 8 precursor antibody
    • CBF-A antibody
    • CCAAT Enhancer Binding Protein alpha antibody
    • CCAAT/enhancer binding protein (C/EBP), alpha antibody
    • CCAAT/enhancer-binding protein alpha antibody
    • CEBP antibody
    • CEBP A antibody
    • CEBP alpha antibody
    • Cebpa antibody
    • CEBPA_HUMAN antibody
    • FADD homologous ICE/CED 3 like protease antibody
    • FADD like ICE antibody
    • FLICE antibody
    • ICE like apoptotic protease 5 antibody
    • ICE8 antibody
    • MACH antibody
    • MCH5 antibody
    • MORT1 associated CED 3 homolog antibody
    see all

图片

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
    All lanes : Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/1000 dilution (Purified)

    Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    This antibody detects high backgrounds

  • Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)
    Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (51)

发表研究结果有使用 ab40764？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40764 被引用在 51 文献中.

  • Zhang Q  et al. Effects of 4-nonylphenol on adipogenesis in 3T3-L1 preadipocytes and C3H/10T1/2 mesenchymal stem cells. J Appl Toxicol 42:588-599 (2022). PubMed: 34553387
  • Li J  et al. Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation. Exp Ther Med 23:81 (2022). PubMed: 34934450
  • Wang Y  et al. AdipoRon exerts opposing effects on insulin sensitivity via fibroblast growth factor 21-mediated time-dependent mechanisms. J Biol Chem 298:101641 (2022). PubMed: 35090894
  • Liang X  et al. Angiotensin II Inhibits Adipogenic Differentiation and Promotes Mature Adipocyte Browning through the Corepressor CtBP1. Biomedicines 10:N/A (2022). PubMed: 36551887
  • Wang L  et al. miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1. Cells 12:N/A (2022). PubMed: 36611960
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

ChIP abreview for Anti-CEBP Alpha/CEBPA antibody [EP709Y]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (B cell)
Negative control
IRF8
Specification
B cell
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
CNPY1
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 28 2023

Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP709Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (fat)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
fat
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 14 2017

Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP709Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (ESC (HUES7))
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Loading amount
10 µg
Specification
ESC (HUES7)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 09 2016

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