重组Anti-CEBP Alpha/CEBPA抗体[EP708Y] (ab40761)
![Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)](https://www.abcam.cn/ps/products/40/ab40761/Images/ab40761-53877-anti-cebp-alpha-antibody-ep708y-western-blot.gif "Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CEBP Alpha/CEBPA抗体[EP708Y]
参阅全部 CEBP Alpha/CEBPA 一抗 -
描述
兔单克隆抗体[EP708Y] to CEBP Alpha/CEBPA -
宿主
Rabbit -
特异性
This antibody recognizes isoform1 and isoform 4.
-
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). The exact sequence is proprietary.
-
阳性对照
- U937+LPS cell lysate, HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP708Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa).
|
| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa). |
|
ICC/IF
1/250 - 1/500. |
靶标
-
功能
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. -
序列相似性
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1050 Human
- Omim: 116897 Human
- SwissProt: P49715 Human
- Unigene: 76171 Human
-
别名
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
图片
-
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg
Predicted band size: 43 kDa
Observed band size: 30,43 kDa why is the actual band size different from the predicted?We are unsure as to the identity of the 30kda band.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)](https://www.abcam.cn/ps/products/40/ab40761/Images/ab40761-53876-anti-cebp-alpha-antibody-ep708y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)ab40761 (1/250) staining HeLa cells using immunofluorescence. -
![Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)](https://www.abcam.cn/ps/products/40/ab40761/Images/ab40761-53875-anti-cebp-alpha-antibody-ep708y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
-
![Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)](https://www.abcam.cn/ps/products/40/ab40761/Images/ab40761-53874-anti-cebp-alpha-antibody-ep708y-western-blot.jpg)
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)This image is courtesy of an anonymous AbreviewAnti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells
Secondary
HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% Milk for 1 hour at 24°C.
We are unsure as to the identity of the 30kda band.
-
![Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)](https://www.abcam.cn/ps/products/40/ab40761/Images/ab40761-5-benefits-of-recombinant-antibodies.png)
Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (18)
ab40761 被引用在 18 文献中.
- Zhang LM et al. CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling. Neoplasma 68:602-612 (2021). PubMed: 33780265
- Ni T et al. Dihydrolycorine Attenuates Cardiac Fibrosis and Dysfunction by Downregulating Runx1 following Myocardial Infarction. Oxid Med Cell Longev 2021:8528239 (2021). PubMed: 34725565
- Pope BD et al. Fattening chips: hypertrophy, feeding, and fasting of human white adipocytes in vitro. Lab Chip 20:4152-4165 (2020). PubMed: 33034335
- Zhang W et al. A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-ß expression. Nutr Metab (Lond) 16:88 (2019). PubMed: 31889968
- Ptasinska A et al. RUNX1-ETO Depletion in t(8;21) AML Leads to C/EBPa- and AP-1-Mediated Alterations in Enhancer-Promoter Interaction. Cell Rep 28:3022-3031.e7 (2019). PubMed: 31533028