Anti-CEACAM20抗体[HT-12D8] (ab59626)
![Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)](https://www.abcam.cn/ps/products/59/ab59626/Images/ab59626-73583-anti-ceacam20-antibody-ht-12d8-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)")
Key features and details
- Mouse monoclonal [HT-12D8] to CEACAM20
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-CEACAM20抗体[HT-12D8] -
描述
小鼠单克隆抗体[HT-12D8] to CEACAM20 -
宿主
Mouse -
特异性
ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20
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阳性对照
- FACS: BOSC23 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab59626 was purified by protein G affinity chromatography from cell culture supernatants. -
克隆
单克隆 -
克隆编号
HT-12D8 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59626于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use 1µg for 106 cells.
|
| 说明 |
|---|
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Flow Cyt
Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use 1µg for 106 cells. |
靶标
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相关性
CEA-related cell adhesion molecule 20 (CEACAM20) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. The CEACAM20 protein contains a single N domain followed by 4 immunoglobulin-like A (A1, A2) and B (B1, B2) domains. Expression of CEACAM20 can be found in tissues of prostate, testis, duodenum and small intestine with highest expression in prostate. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. High expression of CEACAM20 in tissue of prostate carcinoma and in prostate carcinoma cell lines suggest that CEACAM20 can be used as a tumor marker. -
细胞定位
Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 125931 Human
- SwissProt: Q6UY09 Human
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别名
- carcinoembryonic antigen related cell adhesion molecule 20 antibody
- UNQ9366 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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![Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)](https://www.abcam.cn/ps/products/59/ab59626/Images/ab59626-73582-anti-ceacam20-antibody-ht-12d8-flow-cytometry.jpg)
Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).
实验方案
数据表及文件
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Datasheet download
文献 (0)
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