Anti-CEACAM 5 + 6抗体[MUS] (ab4539)
Key features and details
- Mouse monoclonal [MUS] to CEACAM 5 + 6
- Suitable for: IHC-Fr, ELISA, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CEACAM 5 + 6抗体[MUS] -
描述
小鼠单克隆抗体[MUS] to CEACAM 5 + 6 -
宿主
Mouse -
特异性
MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry. -
经测试应用
适用于: IHC-Fr, ELISA, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native Carcino Embryonic Antigen(partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Schozel S et al.).
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常规说明
Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MUS -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 84,41 kDa.
Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 84,41 kDa. Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa |
靶标
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相关性
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. -
细胞定位
Plasma membrane -
数据库链接
- Entrez Gene: 1048 Human
- Entrez Gene: 4680 Human
- Omim: 114890 Human
- Omim: 163980 Human
- SwissProt: P06731 Human
- SwissProt: P40199 Human
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别名
- Carcinoembryonic antigen antibody
- CD66c antibody
- CD66e antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab4539 被引用在 2 文献中.
- Edwards MM et al. Idiopathic preretinal glia in aging and age-related macular degeneration. Exp Eye Res 150:44-61 (2016). PubMed: 26220834
- Tsutsumida H et al. RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells. Clin Cancer Res 12:2976-87 (2006). PubMed: 16707592
