AB5188

Anti-CDYL 抗体

Name: CDYL抗体 (ab5188)| Abcam中文官网
Brand: Abcam Limited
SKU: AB5188
Rating: 4 (2 reviews)

Anti-CDYL antibody

(2 Reviews)

(13 Publications)

Rabbit Polyclonal CDYL antibody. Suitable for ChIP, WB, IHC-P, ChIP-seq and reacts with Mouse, Rat, Vervet monkey, Human samples. Cited in 13 publications.

查看别名

CDYL1, CDYL, Chromodomain Y-like protein, CDY-like, Crotonyl-CoA hydratase

2 Images

Project3835****

Western blot - Anti-CDYL antibody (AB5188)

All lanes:

Western blot - Anti-CDYL antibody (ab5188) at 1 µg/mL

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected at 10 µg

Lane 2:

COS7 CDYL (Human) Transfected Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 66 kDa

Observed band size: 60 kDa,65 kDa,80 kDa

false

Exposure time: 12min

Collaborator

Western blot - Anti-CDYL antibody (AB5188)

Rabbit polyclonal to CDYL (ab5188) at 1/1000.

Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

All lanes:

Western blot - Anti-CDYL antibody (ab5188) at 1/1000 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate

Lane 2:

COS7 cells transfected with HA-CDYL-FLAG plasmid

Predicted band size: 66 kDa

Observed band size: 83 kDa

false

Cecile Lestrat, Saadi Khochbin lab, Grenoble, France

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, African green monkey, Mouse, Rat

应用

IHC-P, WB, ChIP-seq, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "African green monkey": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "predicted", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Vervet monkey": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CDYL known as Chromodomain Y-Like functions as a chromatin modifier protein and has molecular weight around 59 kDa. This protein features a chromodomain that enables it to bind to histone tails influencing gene expression by altering chromatin structure. CDYL expresses broadly in tissues with significant presence in the brain testis and embryonic tissue. It interacts with various protein partners to regulate multiple cellular processes ensuring proper cellular function.

Biological function summary

The role of CDYL revolves around transcriptional repression as it integrates into chromatin-remodeling complexes. This protein contributes to the silencing of gene transcription by compacting chromatin facilitating stable epigenetic inheritance. CDYL works alongside other chromatin-associated proteins and histone deacetylases to maintain transcriptional repression. Its function in these complexes aids in establishing cell identity by regulating gene expression patterns specific to cell type and developmental stage.

Pathways

CDYL integrates into key regulatory networks involved in epigenetic gene silencing and neuronal development. It participates in the chromatin remodeling pathway and is closely associated with the histone modification pathway impacting neuronal differentiation. CDYL interacts with proteins like HDAC1 and G9a methyltransferase coordinating the modification of histones resulting in a variety of epigenetic outcomes. These pathways ensure proper developmental processes and cellular differentiation occur in multicellular organisms.

Researchers associate CDYL with conditions such as neurodevelopmental disorders and cancer. Abnormal expression or mutations in CDYL links to certain cancers including prostate cancer where it interacts with oncogenic proteins influencing cancer progression. Additionally researchers have explored its role in neurological disorders where CDYL's regulation of gene expression affects brain development and function suggesting potential involvement in disorders like schizophrenia. Understanding CDYL's interactions could offer insights into treatment strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 2. Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed : 19808672, PubMed : 28402439). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed : 28402439). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (PubMed : 22009739). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (PubMed : 19061646). Involved in X chromosome inactivation in females : recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (By similarity). Promotes EZH2 accumulation and H3K27me3 methylation at DNA double strand breaks (DSBs), thereby facilitating transcriptional repression at sites of DNA damage and homology-directed repair of DSBs (PubMed : 29177481). Required for neuronal migration during brain development by repressing expression of RHOA (By similarity). By repressing the expression of SCN8A, contributes to the inhibition of intrinsic neuronal excitability and epileptogenesis (By similarity). In addition to acting as a chromatin reader, acts as a hydro-lyase (PubMed : 28803779). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (PubMed : 28803779). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (By similarity). By regulating histone crotonylation and trimethylation of H3K27, may be involved in stress-induced depression-like behaviors, possibly by regulating VGF expression (By similarity).. Isoform 1. Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the presence of a N-terminal extension that inactivates the chromo domain (PubMed : 19808672).. Isoform 3. Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the absence of the chromo domain (PubMed : 19808672). Acts as a negative regulator of isoform 2 by displacing isoform 2 from chromatin.

See full target information CDYL

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 32:1-14 PubMed33175657

2020

EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tian,Timothy Billings,Petko M Petkov

Journal of proteome research 19:1533-1547 PubMed32159963

2020

Proteomic Analyses Identify a Novel Role for EZH2 in the Initiation of Cancer Cell Drug Tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Pham,Robert Pitti,Charles A Tindell,Tommy K Cheung,Alexandre Masselot,Jean-Phillipe Stephan,Gulfem D Guler,Catherine Wilson,Jennie Lill,David Arnott,Marie Classon

Theranostics 9:4717-4729 PubMed31367252

2019

CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengang Qiu,Weiliang Zhu,Hui Meng,Lihua Tong,Xi Li,Peng Luo,Lilan Yi,Xiaoli Zhang,Linlang Guo,Ting Wei,Jian Zhang

Cell 175:1887-1901.e18 PubMed30550787

2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Leyun Wang,Guihai Feng,Yukai Wang,Yufei Li,Xin Li,Chao Liu,Guanyi Jiao,Cheng Huang,Junchao Shi,Tong Zhou,Qi Chen,Zhonghua Liu,Wei Li,Qi Zhou

Oncogene 37:461-477 PubMed28967906

2017

EZH2 promotes neoplastic transformation through VAV interaction-dependent extranuclear mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

N Venkatesan,J F Wong,K P Tan,H H Chung,Y H Yau,E Cukuroglu,A Allahverdi,L Nordenskiöld,J Göke,S Geifman-Shochat,V C L Lin,M S Madhusudhan,I-H Su

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:4494-508 PubMed24671995

2014

Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2.

Applications

Unspecified application

Species

Rat

Cai Qi,Shumeng Liu,Rui Qin,Yu Zhang,Guoqiang Wang,Yongfeng Shang,Yun Wang,Jing Liang

Molecular and cellular biology 33:5005-20 PubMed24144980

2013

Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

M Escamilla-Del-Arenal,S T da Rocha,C G Spruijt,O Masui,O Renaud,Arne H Smits,R Margueron,M Vermeulen,E Heard

The Journal of biological chemistry 286:42414-25 PubMed22009739

2011

Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.

Applications

ChIP-seq, ChIP, WB

Species

Human, Human, Human

Yu Zhang,Xiaohan Yang,Bin Gui,Guojia Xie,Di Zhang,Yongfeng Shang,Jing Liang

BMC systems biology 5:136 PubMed21867514

2011

MicroRNAs coordinately regulate protein complexes.

Applications

Species

Human

Steffen Sass,Sabine Dietmann,Ulrike C Burk,Simone Brabletz,Dominik Lutter,Andreas Kowarsch,Klaus F Mayer,Thomas Brabletz,Andreas Ruepp,Fabian J Theis,Yu Wang

Nature biotechnology 29:255-65 PubMed21258344

2011

Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Marcus Bantscheff,Carsten Hopf,Mikhail M Savitski,Antje Dittmann,Paola Grandi,Anne-Marie Michon,Judith Schlegl,Yann Abraham,Isabelle Becher,Giovanna Bergamini,Markus Boesche,Manja Delling,Birgit Dümpelfeld,Dirk Eberhard,Carola Huthmacher,Toby Mathieson,Daniel Poeckel,Valérie Reader,Katja Strunk,Gavain Sweetman,Ulrich Kruse,Gitte Neubauer,Nigel G Ramsden,Gerard Drewes

View all publications

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Anti-CDYL 抗体

Anti-CDYL antibody

Western blot - Anti-CDYL antibody (AB5188)

Western blot - Anti-CDYL antibody (AB5188)