重组Anti-CDX2抗体[EPR2764Y] (ab76541)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 ( human colorectal adenocarcinoma epithelial cell) labelling CDX2 with ab76541 at 1/400 (1.82 μg/ml) dilution (Green).

Negative control: MCF7.

Confocal image showing nuclear staining on the SW480 cell line.

Nuclear DNA was labelled with DAPI (shown in blue).

Secondary used was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 (2.5μg/ml) dilution.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unpurified ab76541 staining CDX2 in human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

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Unpurified ab76541 showing positive staining in normal colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541 showing positive staining in colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541 showing negative staining in skeletal muscle tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541 showing negative staining in normal kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.