Anti-CDX2抗体(ab48009)
Key features and details
- Goat polyclonal to CDX2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CDX2抗体
参阅全部 CDX2 一抗 -
描述
山羊多克隆抗体to CDX2 -
宿主
Goat -
特异性
No crossreactivity expected with CDX1 or CDX4. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Hamster -
免疫原
Synthetic peptide:
EQLSPGGQRRNLCE
, corresponding to internal sequence amino acids 154-167 of Human CDX2. -
阳性对照
- WB: Human colon lysate; IHC-P: Human colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48009于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P | (1) |
Use a concentration of 5 µg/ml.
|
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. -
序列相似性
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
翻译后修饰
Phosphorylation of Ser-60 mediates the transactivation capacity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 374205 Chicken
- Entrez Gene: 101841392 Hamster
- Entrez Gene: 1045 Human
- Entrez Gene: 12591 Mouse
- Entrez Gene: 66019 Rat
- Omim: 600297 Human
- SwissProt: Q04649 Hamster
- SwissProt: Q99626 Human
see all -
别名
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
see all
图片
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Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).
Predicted band size: 34 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. The observed 35kDa band was successfully blocked by incubation with the immunizing peptide.
数据表及文件
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Datasheet download
文献 (1)
ab48009 被引用在 1 文献中.
- Liu X et al. A novel case of endometrial dedifferentiated adenocarcinoma associated with MLH1 promotor hypermethylation and microsatellite instability. Pathol Res Pract 214:1904-1908 (2018). PubMed: 30173944