Anti-CDKN2A/p19ARF抗体(ab80)
Key features and details
- Rabbit polyclonal to CDKN2A/p19ARF
- Suitable for: ICC/IF, IHC-Fr, ELISA, ICC, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CDKN2A/p19ARF抗体
参阅全部 CDKN2A/p19ARF 一抗 -
描述
兔多克隆抗体to CDKN2A/p19ARF -
宿主
Rabbit -
特异性
On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs. -
经测试应用
适用于: ICC/IF, IHC-Fr, ELISA, ICC, WB, IHC-Pmore details -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
常规说明
A monoclonal alternative to this target is available (ab26696).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC |
Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle).
|
|
| WB | (7) |
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples.
|
| IHC-P | (2) |
Use at an assay dependent concentration. PubMed: 11726500
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC
Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). |
|
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 11726500 |
靶标
-
相关性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
-
别名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
图片
-
Western blot - Anti-CDKN2A/p19ARF antibody (ab80)This image is courtesy of Charles SherrAll lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/ml
Lanes 1-2 : mouse embryonic fibroblast total cell extract.
Lane 3 : mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDaWestern blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 µg/ml. (30 µg of protein from total cell extracts per lane.)
-
Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)Pictures were generously provided by Dr. David Bertwistleab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).
Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (212)
ab80 被引用在 212 文献中.
- Bazzar W et al. Pharmacological inactivation of CDK2 inhibits MYC/BCL-XL-driven leukemia in vivo through induction of cellular senescence. Cell Cycle 20:23-38 (2021). PubMed: 33356836
- Chen P et al. Intramuscular injection of Botox causes tendon atrophy by induction of senescence of tendon-derived stem cells. Stem Cell Res Ther 12:38 (2021). PubMed: 33413592
- Li Y et al. Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication. NPJ Precis Oncol 5:14 (2021). PubMed: 33654169
- Kotowski K et al. 3PO as a Selective Inhibitor of 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells. Anticancer Res 40:2613-2625 (2020). PubMed: 32366406
- Mikawa R et al. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules 10:N/A (2020). PubMed: 32192082