Anti-CDKN2A/p19ARF抗体[5-C3] - BSA and Azide free (ab174939)
Key features and details
- Rat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free
- Suitable for: IHC-FoFr, WB, IP, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-CDKN2A/p19ARF抗体[5-C3] - BSA and Azide free
参阅全部 CDKN2A/p19ARF 一抗 -
描述
大鼠单克隆抗体[5-C3] to CDKN2A/p19ARF - BSA and Azide free -
宿主
Rat -
经测试应用
适用于: IHC-FoFr, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse
不与反应: Hamster, Human -
免疫原
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
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阳性对照
- 3T3 cell lystate positive control.
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常规说明
ab174939 is the carrier-free version of ab26696.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
5-C3 -
骨髓瘤
Y3/Ag1.2.3 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-FoFr |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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IHC-FoFr
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
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别名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)
This ICC/IF data was generated using the same antibody clone in a different buffer formulation (ab26696).
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab174939 被引用在 1 文献中.
- Millar FR et al. Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer. Cell Rep 41:111596 (2022). PubMed: 36351380