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重组RabMAb

重组Anti-CDKN2A/p14ARF抗体[SP271] - BSA and Azide free (ab271999)

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Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
  • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
  • Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP271] to CDKN2A/p14ARF - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 750 Unconjugated

概述

  • 产品名称

    Anti-CDKN2A/p14ARF抗体[SP271] - BSA and Azide free
    参阅全部 CDKN2A/p14ARF 一抗

  • 描述

    兔单克隆抗体[SP271] to CDKN2A/p14ARF - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC: Human cervical squamous cell carcinoma tissue. WB: HeLa cell lysate. ICC/IF: HeLa and MCF7 cells. Flow Cyt (intra): HEK293 and HeLa cells.

  • 常规说明

    ab271999 is the carrier-free version of ab216602.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271999于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

(Incubate for 30 minutes at 4°C).
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

(Incubate for 1 hour at room temperature).
IHC-P
Use at an assay dependent concentration.

(Primary incubation for 10 minutes at room temperature).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

(Incubate for 30 minutes at 4°C).
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

(Incubate for 1 hour at room temperature).
IHC-P
Use at an assay dependent concentration.

(Primary incubation for 10 minutes at room temperature).

靶标

  • 相关性

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.

  • 细胞定位

    Cytoplasmic and Nuclear

  • 数据库链接

    • 别名

      • ARF antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A antibody
      • INK4 antibody
      • INK4a antibody
      • Melanoma p16 inhibits CDK4 antibody
      • MLM antibody
      • MTS 1 antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • p16 INK4a antibody
      • p16INK4a antibody
      • p19 antibody
      • P19ARF antibody
      • TP16 antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
      Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/420 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
      Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF  with purified ab216602 at 1/500 (0.86 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma labeling CDKN2A/p14ARF with ab216602 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).
    • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
      Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

      Intracellular flow cytometric analysis of HEK293 cells labeling CDKN2A/p14ARF with ab216602 at 1/400 dilution (green) compared to negative control rabbit IgG (blue) . 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602). 

       

       

    • Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
      Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271999？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271999 尚未被引用在任何文献中。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com