Anti-Cdk9 (phospho T186)抗体(ab79178)
Key features and details
- Rabbit polyclonal to Cdk9 (phospho T186)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Cdk9 (phospho T186)抗体
参阅全部 Cdk9 一抗 -
描述
兔多克隆抗体to Cdk9 (phospho T186) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human Cdk9 (phospho T186).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79178于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa. |
靶标
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功能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
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别名
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
图片
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Western blot - Anti-Cdk9 (phospho T186) antibody (ab79178)All lanes : Anti-Cdk9 (phospho T186) antibody (ab79178)
Lane 1 : 3T3 cells treated with Forskolin (40 nM, 30 minutes)
Lane 2 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) with phospho peptide
Predicted band size: 43 kDa
Observed band size: 43 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab79178 被引用在 3 文献中.
- Huang Y et al. Human cytomegalovirus RNA2.7 inhibits RNA polymerase II (Pol II) Serine-2 phosphorylation by reducing the interaction between Pol II and phosphorylated cyclin-dependent kinase 9 (pCDK9). Virol Sin 37:358-369 (2022). PubMed: 35537980
- Li J et al. CDK9 inhibitor CDKI-73 is synergetic lethal with PARP inhibitor olaparib in BRCA1 wide-type ovarian cancer. Am J Cancer Res 10:1140-1155 (2020). PubMed: 32368391
- Pinto DO et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006