AB6544

Anti-Cdk9 抗体

Name: Cdk9抗体 (ab6544)| Abcam中文官网
Brand: Abcam Limited
SKU: AB6544
Rating: 3 (5 reviews)

Anti-Cdk9 antibody

(5 Reviews)

(12 Publications)

Rabbit Polyclonal CDK9 antibody. Suitable for WB, IHC-P, ELISA, IP and reacts with Human, Mouse samples. Cited in 12 publications.

查看别名

CDC2L4, TAK, CDK9, Cyclin-dependent kinase 9, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (AB6544)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (AB6544)

Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.

Supplier Data

Western blot - Anti-Cdk9 antibody (AB6544)

All lanes:

Western blot - Anti-Cdk9 antibody (ab6544) at 1/1000 dilution

Lane 1:

Opal PreStain 11-245 kDa (MB-210-0500) at 10 µL

Lane 2:

Human kidney lysate at 5 µg

Lane 3:

PC3 lysate at 20 µg

Lane 4:

Blank well

Predicted band size: 43 kDa

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Exposure time: 46s

CiteAb

Western blot - Anti-Cdk9 antibody (AB6544)

Cdk9 western blot using anti-Cdk9 antibody ab6544. Publication image and figure legend from Kuzmina, A., Verstraete, N., et al., 2014, Retrovirology, PubMed 24985467.

ab6544 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab6544 please see the product overview.

Association of HA-CycT1-V107E with P-TEFb interacting partners and its RNA targets. (a + b)HA-CycT1-V107E mutant does not bind TAR or 7SK snRNA in cells – HEK-293T cells were co-transfected with HA-CycT1-V107E mutant, or HA-CycT1-wild type, HIV LTR-Luciferase and pCDNA-Myc-Tat plasmids. 48 hr. post transfection, cells were lysed and immuno-precipitated (IP) with either anti-HA antibody, or control non-immune anti-human IgG. RNA was extracted from IP and input samples (1%) and was then subjected to cDNA synthesis, which was further analyzed by real time PCR using 7SK-snRNA (a) and TAR specific primers (b). Reactions were analyzed by real time PCR in triplicates and presented as fold of mean enrichment relatively to PCR results obtained for cells transfected with LTR-Luciferase alone - set to 1. Error bars show ± SEM values. (c)Association of HA-CycT1-V107E mutants with P-TEFb in cells – HEK-293T cells stably expressing either HA-CycT1-wild type or HA-V107E-CycT1 were co-transfected with Flag-Tat using lipofectamin 2000 (Invitrogen). 48 hr. post transfection, cells were lysed and subjected to IP with α-Flag antibody. IP reactions were analyzed by WB with a Cdk9 antibody. α-HA WB represents 1% of input of HA-CycT1. (d)Association of HA-CycT1-V107E mutants with P-TEFb transcription partners in cells – HEK-293T cells stably expressing either HA-CycT1-wild type or HA-V107E-CycT1 were lysed and subjected to IP with α-HA (left panel). IP reactions were analyzed by WB with the indicated antibodies.

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

ELISA, IHC-P, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Antiserum will specifically react with a 43 kDa cdk9 (PITALRE) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human. For immunohistochemistry use paraffin embedded tissue.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "" } } }

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产品详情

cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.

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性能和储存信息

形式

Liquid

纯度

Whole antiserum

纯化说明

Sterile filtered.

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cyclin-dependent kinase 9 (Cdk9) is a serine/threonine protein kinase involved in regulating transcription elongation. It is often referred to as part of the positive transcription elongation factor b (P-TEFb) complex. Cdk9 partners with cyclin T1 T2 or K to form this complex allowing it to phosphorylate the carboxyl-terminal domain (CTD) of RNA polymerase II. The molecular weight of Cdk9 is around 42 kDa. This protein is ubiquitously expressed found in many tissues including the heart liver and brain reflecting its important role.

Biological function summary

Cdk9 functions as a central component of transcriptional processes. It is essential for the transition of RNA polymerase II from the initiation phase to productive elongation. As part of the P-TEFb complex Cdk9 targets negative elongation factors helping relieve their inhibition. This regulation is especially important in the control of genes with strong promoter proximally paused polymerase. By doing so Cdk9 controls the expression of several genes involved in cell growth differentiation and response to stress.

Pathways

Cdk9 plays a significant role in transcription and signal transduction pathways. One important pathway is the NF-κB signaling where Cdk9 regulates the transcription of NF-κB target genes involved in immune response. Another significant pathway is the heat shock response where Cdk9 enhances the expression of heat shock proteins helping cells deal with stress. In both pathways it cooperates with other proteins like cyclins and negative elongation factors illustrating its interconnected role in cellular function.

Cdk9 has been linked to several conditions most notably cancer and cardiac hypertrophy. Its overactivation can lead to increased transcription of genes involved in proliferation making it a focus for cancer research. Cdk9 is also implicated in cardiac hypertrophy where its activity affects gene expression that can exacerbate the condition. Researchers study its interaction with other proteins such as THZ1 a Cdk9 inhibitor to explore potential therapeutic avenues.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein kinase involved in the regulation of transcription (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094, PubMed : 29335245). Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094, PubMed : 30134174). This complex is inactive when in the 7SK snRNP complex form (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094). Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE (PubMed : 10912001, PubMed : 11112772, PubMed : 12037670, PubMed : 20081228, PubMed : 20980437, PubMed : 21127351, PubMed : 9857195). Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling) (PubMed : 17956865, PubMed : 18362169). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis (PubMed : 10393184, PubMed : 11112772). P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export (PubMed : 15564463, PubMed : 19575011, PubMed : 19844166). Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing (PubMed : 15564463, PubMed : 19575011, PubMed : 19844166). The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro (PubMed : 21127351). Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage (PubMed : 20493174). In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6 (PubMed : 20493174). Promotes cardiac myocyte enlargement (PubMed : 20081228). RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription (PubMed : 21127351). AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect (PubMed : 10912001, PubMed : 11112772, PubMed : 9857195). The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (PubMed : 12037670). Catalyzes phosphorylation of KAT5, promoting KAT5 recruitment to chromatin and histone acetyltransferase activity (PubMed : 29335245).

See full target information CDK9

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012172 PubMed38662769

2024

Direct and indirect effects of CYTOR lncRNA regulate HIV gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alona Kuzmina,Lopamudra Sadhu,Md Hasanuzzaman,Koh Fujinaga,Jacob C Schwartz,Oliver T Fackler,Ran Taube

Nature communications 14:378 PubMed36690674

2023

Structural mechanism of BRD4-NUT and p300 bipartite interaction in propagating aberrant gene transcription in chromatin in NUT carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Di Yu,Yingying Liang,Claudia Kim,Anbalagan Jaganathan,Donglei Ji,Xinye Han,Xuelan Yang,Yanjie Jia,Ruirui Gu,Chunyu Wang,Qiang Zhang,Ka Lung Cheung,Ming-Ming Zhou,Lei Zeng

Molecular cell 82:1724-1736.e7 PubMed35320752

2022

Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yang,Shiheng Liu,Sylvain Egloff,Catherine D Eichhorn,Tanya Hadjian,James Zhen,Tamás Kiss,Z Hong Zhou,Juli Feigon

Molecular cell 81:2944-2959.e10 PubMed34166609

2021

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Skalska,Victoria Begley,Manuel Beltran,Saulius Lukauskas,Garima Khandelwal,Peter Faull,Amandeep Bhamra,Manuel Tavares,Rachel Wellman,Andrey Tvardovskiy,Benjamin M Foster,Igor Ruiz de Los Mozos,Javier Herrero,Silvia Surinova,Ambrosius P Snijders,Till Bartke,Richard G Jenner

Retrovirology 17:6 PubMed32228614

2020

DDX5 potentiates HIV-1 transcription as a co-factor of Tat.

Applications

Unspecified application

Species

Unspecified reactive species

Nyaradzai Sithole,Claire A Williams,Truus E M Abbink,Andrew M L Lever

AIDS research and human retroviruses 35:710-717 PubMed31044597

2019

HIV Transcription Is Independent of Mediator Kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele C Cary,Mona Rheinberger,Ajda Rojc,B Matija Peterlin

Nucleic acids research 44:9719-9732 PubMed27471030

2016

The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation.

Applications

Unspecified application

Species

Unspecified reactive species

Jixiu Shan,Fan Zhang,Jason Sharkey,Tiffany A Tang,Tönis Örd,Michael S Kilberg

Retrovirology 11:51 PubMed24985467

2014

A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription.

Applications

Species

Unspecified reactive species

Alona Kuzmina,Nina Verstraete,Sigal Galker,Maayan Maatook,Olivier Bensaude,Ran Taube

Proceedings of the National Academy of Sciences of 110:13546-51 PubMed23898190

2013

SIRT2 directs the replication stress response through CDK9 deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Seong-Hoon Park,Brooke G Pantazides,Oleksandra Karpiuk,Matthew D Warren,Claire W Hardy,Duc M Duong,So-Jeong Park,Hyun-Seok Kim,Athanassios Vassilopoulos,Nicholas T Seyfried,Steven A Johnsen,David Gius,David S Yu

The Journal of biological chemistry 286:15171-81 PubMed21378166

2011

G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb).

Applications

ICC/IF

Species

Human

Tianyang Qi,Wen Tang,Ling Wang,Lei Zhai,Lijing Guo,Xianlu Zeng

View all publications

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Anti-Cdk9 抗体

Anti-Cdk9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (AB6544)

Western blot - Anti-Cdk9 antibody (AB6544)

Western blot - Anti-Cdk9 antibody (AB6544)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性