Anti-Cdk8抗体(ab272879)
Key features and details
- Rabbit polyclonal to Cdk8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Cdk8抗体
参阅全部 Cdk8 一抗 -
描述
兔多克隆抗体to Cdk8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Cdk8 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P49336 -
阳性对照
- WB: A549, H1299 and HCT 116 whole cell lysate. IHC-P: Mouse testis tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272879于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1024 Human
- Entrez Gene: 264064 Mouse
- Omim: 603184 Human
- SwissProt: P49336 Human
- SwissProt: Q8R3L8 Mouse
- Unigene: 382306 Human
- Unigene: 260576 Mouse
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别名
- cdk8 antibody
- CDK8 protein kinase antibody
- CDK8_HUMAN antibody
see all
图片
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Western blot - Anti-Cdk8 antibody (ab272879)All lanes : Anti-Cdk8 antibody (ab272879) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk8 antibody (ab272879)Paraffin-embedded mouse testis tissue stained for CDK8 using ab272879 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272879 被引用在 1 文献中.
- Zhou B et al. CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis. Drug Dev Res 82:1131-1143 (2021). PubMed: 33818788