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AB272879

Anti-Cdk8抗体

Anti-Cdk8 antibody

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(1 Publication)

Rabbit Polyclonal CDK8 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CDK8.

查看别名

Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, CDK8

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk8 antibody (AB272879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk8 antibody (AB272879)

Paraffin-embedded mouse testis tissue stained for CDK8 using ab272879 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human CDK8. The exact immunogen used to generate this antibody is proprietary information.

P49336

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搭配使用产品

Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB198843

Anti-CDC2L6 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC2L6 antibody - N-terminal (AB198843)

  • 0
  • 0
Primary Antibodies

AB76923

Anti-MED13/GCT antibody

Immunoprecipitation - Anti-MED13/GCT antibody (AB76923)

  • 0
  • 0
Primary Antibodies

AB300575

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [EPR24494-59] (ChIP Grade)

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [EPR24494-59] (ChIP Grade) (AB300575)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cdk8 also known as Cyclin-dependent kinase 8 forms an important component of the transcription regulation machinery. It has a molecular mass of about 66 kDa and is widely expressed in various tissues though its abundance can vary. Cdk8 operates largely in the nucleus of cells where it participates in regulating gene expression. Cdk8 acts as a kinase by modifying other proteins through phosphorylation which alters their activity and function.
Biological function summary

Cdk8 plays a critical role in modulating transcriptional responses. It does this by becoming part of the Mediator complex which is essential for transcription regulation by RNA Polymerase II. The Mediator complex where Cdk8 acts as a kinase influences the initiation and regulation of gene expression. Cdk8 phosphorylates several transcription factors and other components affecting their ability to activate or repress transcription.

Pathways

Cyclin-dependent kinase 8 functions prominently in the Wnt/β-catenin signaling and Notch signaling pathways both essential for development and cell differentiation. In the Wnt pathway it interacts closely with proteins like β-catenin to influence gene activation. In the Notch pathway it modulates transcription factors related to cellular differentiation processes. These interactions underline its role as a regulator in carefully managing cellular responses to signaling cues.

Cdk8 has been implicated in cancer and congenital disorders. Its overactivity can lead to uncontrolled cell growth contributing to tumor progression notably in colorectal cancer where it affects genes in the Wnt pathway. Beyond cancer its dysfunction links to congenital heart defects where it affects cardiogenesis-associated proteins such as NOTCH1. Cdk8’s involvement in these pathways highlights its part in both regular development and disease pathogenesis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
See full target information CDK8
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 82:1131-1143 PubMed33818788

2021

CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhou,Ting Li,Rongping Xie,Jianbin Zhou,Jue Liu,Yu Luo,Xia Zhang
View all publications
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