Anti-Cdk6 (phospho Y13)抗体(ab131439)
Key features and details
- Rabbit polyclonal to Cdk6 (phospho Y13)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdk6 (phospho Y13)抗体
参阅全部 Cdk6 一抗 -
描述
兔多克隆抗体to Cdk6 (phospho Y13) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human Cdk6 (phospho Y13) conjugated to keyhole limpet haemocyanin.
Database link: Q00534 -
阳性对照
- 293 cell extract treated with Hydroxyurea; HeLa cells; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131439 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131439于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
|
IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1021 Human
- Entrez Gene: 114483 Rat
- Omim: 603368 Human
- SwissProt: Q00534 Human
- Unigene: 119882 Human
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别名
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
图片
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Western blot - Anti-Cdk6 (phospho Y13) antibody (ab131439)All lanes : Anti-Cdk6 (phospho Y13) antibody (ab131439) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with Hydroxyurea
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 (phospho Y13) antibody (ab131439)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cdk6 (phospho Y13) with ab131439 at 1/50 dilution. Right panel was preincubated with blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 (phospho Y13) antibody (ab131439)Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y13) with ab131439 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131439 被引用在 3 文献中.
- Weiss A et al. Targeting cyclin-dependent kinases for the treatment of pulmonary arterial hypertension. Nat Commun 10:2204 (2019). PubMed: 31101827
- Yang S et al. MicroRNA-4712-5p promotes proliferation of the vulvar squamous cell carcinoma cell line A431 by targeting PTEN through the AKT/cyclin D1 signaling pathways. Oncol Rep 42:1689-1698 (2019). PubMed: 31545465
- Liu Q et al. Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide. Exp Ther Med 14:4263-4271 (2017). PubMed: 29075340