Name: Anti-CDK5 antibody [EP715Y]
Brand: RabMAb
SKU: ab40773
Rating: 4 (7 reviews)

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Immunoprecipitation - Anti-CDK5 antibody [EP715Y] (ab40773)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP715Y] to CDK5
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

产品名称

Anti-CDK5抗体[EP715Y]
参阅全部 CDK5 一抗
描述

兔单克隆抗体[EP715Y] to CDK5
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details
种属反应性

与反应: Mouse, Rat, Human
预测可用于: Chicken
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa whole cell lysate (ab150035), NIH3T3 whole cell lysate, PC-12 whole cell lysate ICC/IF: SH-SY5Y (Human neuroblastoma epithelial cell)
常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP715Y
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant Human Cdk5 protein (ab56282)

The Abpromise guarantee

Abpromise™承诺保证使用ab40773于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(5)	1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP		1/20.
ICC/IF		1/50.

说明
Flow Cyt (Intra) 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
ICC/IF 1/50.

功能

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
序列相似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
细胞定位

Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
Target information above from: UniProt accession Q00535 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 100190948 Chicken
- Entrez Gene: 1020 Human
- Entrez Gene: 12568 Mouse
- Entrez Gene: 140908 Rat
- Omim: 123831 Human
- SwissProt: Q00535 Human
- SwissProt: P49615 Mouse
- SwissProt: Q03114 Rat
- Unigene: 647078 Human
- Unigene: 298798 Mouse
- Unigene: 10749 Rat
see all
别名
- Cdk 5 antibody
- Cdk5 antibody
- CDK5_HUMAN antibody
- Cell division protein kinase 5 antibody
- Crk6 antibody
- Cyclin dependent kinase 5 antibody
- Cyclin-dependent kinase 5 antibody
- Protein kinase CDK5 splicing antibody
- PSSALRE antibody
- Serine threonine protein kinase PSSALRE antibody
- Serine/threonine-protein kinase PSSALRE antibody
- Tau protein kinase II catalytic subunit antibody
- TPKII catalytic subunit antibody
see all

Immunoprecipitation - Anti-CDK5 antibody [EP715Y] (ab40773)

Purified ab40773 at 1:20 dilution (1µg) immunoprecipitating CDK5 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg.
Lane 2 (+): ab40773 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40773 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)™(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 33 kDa
Flow Cytometry (Intracellular) - Anti-CDK5 antibody [EP715Y] (ab40773)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling CDK5 with Purified ab40773 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-CDK5 antibody [EP715Y] (ab40773)

All lanes : Anti-CDK5 antibody [EP715Y] (ab40773) at 1/2000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-CDK5 antibody [EP715Y] (ab40773)

Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CDK5 with Purified ab40773 at 1/50 dilution (4.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Cdk5 antibody [EP715Y] (ab40773)

All lanes : Anti-CDK5 antibody [EP715Y] (ab40773) at 1/2000 dilution (Unpurified)

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CDK5 knockout HAP1 whole cell lysate
Lane 3 : SHSY5Y whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab40773 observed at 33 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab40773 was shown to specifically react with Cdk5 in wild-type HAP1 cells as signal was lost in CDK5 knockout cells. Wild-type and CDK5 knockout samples were subjected to SDS-PAGE. Ab40773 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-CDK5 antibody [EP715Y] (ab40773)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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发表研究结果有使用 ab40773？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40773 被引用在 34 文献中.

Zhou H et al. Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice. Mol Brain 15:53 (2022). PubMed: 35701839
Song L et al. Sigma non-opioid receptor 1 is a potential therapeutic target for long QT syndrome. Nat Cardiovasc Res 1:142-156 (2022). PubMed: 36051854
Pan Y et al. Ring finger protein 126 promotes breast cancer metastasis and serves as a potential target to improve the therapeutic sensitivity of ATR inhibitors. Breast Cancer Res 24:92 (2022). PubMed: 36539893
Hu D et al. Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Nat Commun 12:5305 (2021). PubMed: 34489447
Zhang X et al. Inhibition of Cdk5 in PV Neurons Reactivates Experience-Dependent Plasticity in Adult Visual Cortex. Int J Mol Sci 23:N/A (2021). PubMed: 35008611

View all Publications for this product

客户评价及客户问答

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提交评价提交问题

1-8 of 8 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (PANC-1 cells)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

35 µg

Specification

PANC-1 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 30 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Sheep Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Yes - 70% Ethanol O/N at 4C

Specification

Brain

Blocking step

Dako Block #X0909 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 23 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Chicken Tissue (Midbrain cryosection)

Permeabilization

Yes - 0.1% PBS Triton X

Specification

Midbrain cryosection

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 12 2015

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (10% SDS gel)

Sample

Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))

Specification

human iPS cell derived neurons (healthy/diseased)

Treatment

quinolinic acid 72h (and non treated) 500nM

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 17 2014

Application

Western blot

Loading amount

40 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - whole cell (HeLa cell line)

Specification

HeLa cell line

Blocking step

BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 12 2013

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing

Sample

Human Cell lysate - whole cell (U2OS cells)

Specification

U2OS cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 12 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK 293)

Loading amount

20 µg

Specification

HEK 293

Gel Running Conditions

Non-reduced Denaturing (12.5)

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 22 2011

Question

I would like to test ab40773 for use in IHC-FoFr.

Abcam community

Verified customer

Asked on Dec 01 2011

Answer

DISCOUNT CODE: *** Expiration date: April 1st, 2012 I am very pleased to hear you would like to accept our offer and test ab40773 in IHC-FoFr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-FoFr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

回复于 Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (34)

客户评价及客户问答

Western blot abreview for Anti-CDK5 antibody [EP715Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cdk5 antibody [EP715Y]

Immunohistochemistry (Frozen sections) abreview for Anti-Cdk5 antibody [EP715Y]

Western blot abreview for Anti-Cdk5 antibody [EP715Y]

Western blot abreview for Anti-Cdk5 antibody [EP715Y]

Western blot abreview for Anti-Cdk5 antibody [EP715Y]

Western blot abreview for Anti-Cdk5 antibody [EP715Y]