重组Anti-CDK2 (phospho T160) + CDK1 (phospho T161)抗体[EPR17621] (ab183554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17621] to CDK1 (phospho T161) + CDK2 (phospho T160)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CDK2 (phospho T160) + CDK1 (phospho T161)抗体[EPR17621] -
描述
兔单克隆抗体[EPR17621] to CDK1 (phospho T161) + CDK2 (phospho T160) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17621 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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Dot blot |
1/1000.
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说明 |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Dot blot
1/1000. |
靶标
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细胞定位
CDK1: Nucleus. -
数据库链接
- Entrez Gene: 1017 Human
- Entrez Gene: 983 Human
- Omim: 116940 Human
- Omim: 116953 Human
- SwissProt: P06493 Human
- SwissProt: P24941 Human
- Unigene: 19192 Human
- Unigene: 334562 Human
see all -
形式
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
图片
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All lanes : Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 40 ng/ml nocodazole for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
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Dot blot analysis of CDK2 (phospho T160) phospho peptide (Lane 1) and CDK2 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK2 (phospho T160) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Dot blot analysis of CDK1 (phospho T161) phospho peptide (Lane 1) and CDK1 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK1 (phospho T161) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab183554 被引用在 1 文献中.
- Cui D et al. Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways. Cancer Biol Ther N/A:1-9 (2018). PubMed: 30404567