CDK12重组抗体[EPR29009-30]_CDK12抗体(ab317746)| Abcam中文官网

产品名称

Anti-CDK12抗体[EPR29009-30]
参阅全部 CDK12 一抗
描述

兔单克隆抗体[EPR29009-30] to CDK12
宿主

Rabbit
经测试应用

适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: 293T, 293T transfected with siRNA specifically targeting CDK12, HeLa, 293T, K-562, 293T, K-562, NIH/3T3, PC-12, Mouse testis and Rat testis lysates. IHC-P: Human stomach, Human testis, Human colon carcinoma, Mouse colon, Mouse cerebrum, Rat colon and Rat testis tissues. IP: 293T cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR29009-30
同种型

IgG
研究领域

Alternative Versions
- Anti-CDK12 antibody [EPR29009-30] - BSA and Azide free (ab317747)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
HRP detection kit
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-Vinculin antibody [EPR8185] (ab129002)

The Abpromise guarantee

Abpromise™承诺保证使用ab317746于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000. Predicted molecular weight: 164 kDa.
IP		1/30.
IHC-P		1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
WB 1/1000. Predicted molecular weight: 164 kDa.
IP 1/30.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

应用说明

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

功能

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
组织特异性

Ubiquitously expressed.
疾病相关

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
序列相似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
翻译后修饰

Phosphorylation at Thr-893 increases kinase activity.
细胞定位

Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
Target information above from: UniProt accession Q9NYV4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 51755 Human
- Entrez Gene: 69131 Mouse
- Entrez Gene: 192350 Rat
- Omim: 615514 Human
- SwissProt: Q9NYV4 Human
- SwissProt: Q14AX6 Mouse
- SwissProt: Q3MJK5 Rat
- Unigene: 416108 Human
- Unigene: 260516 Mouse
- Unigene: 486492 Mouse
- Unigene: 488484 Mouse
- Unigene: 48817 Rat
see all
别名
- arginine/serine-rich antibody
- Cdc2 related kinase arginine/serine rich antibody
- CDC2 related protein kinase 7 antibody
- Cdc2-related kinase antibody
- CDC2-related protein kinase 7 antibody
- Cdk12 antibody
- CDK12_HUMAN antibody
- Cell division cycle 2 related protein kinase 7 antibody
- Cell division cycle 2-related protein kinase 7 antibody
- Cell division protein kinase 12 antibody
- CRK7 antibody
- CRKR antibody
- CrkRS antibody
- Cyclin-dependent kinase 12 antibody
- EC 2.7.11.22 antibody
- hCDK12 antibody
- kiaa0904 antibody
- Pksc antibody
see all

Western blot - Anti-CDK12 antibody [EPR29009-30] (ab317746)

All lanes : Anti-CDK12 antibody [EPR29009-30] (ab317746) at 1/1000 dilution

Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : 293T transfected with siRNA specifically targeting CDK12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 164 kDa
Observed band size: 205 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
Western blot - Anti-CDK12 antibody [EPR29009-30] (ab317746)

All lanes : Anti-CDK12 antibody [EPR29009-30] (ab317746) at 1/1000 dilution

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 4 : HeLa (human cervical adenocarcinoma epithelial cell) whole fresh cell lysate
Lane 5 : 293T (human embryonic kidney epithelial cell) whole fresh cell lysate
Lane 6 : K-562 (human chronic myelogenous leukemia lymphoblast) whole fresh cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 164 kDa
Observed band size: 205 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In lanes 1-3, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (205 kDa) are likey to be degradation products. In lanes 4-6, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
Western blot - Anti-CDK12 antibody [EPR29009-30] (ab317746)

All lanes : Anti-CDK12 antibody [EPR29009-30] (ab317746) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 164 kDa
Observed band size: 205 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Western blot - Anti-CDK12 antibody [EPR29009-30] (ab317746)

All lanes : Anti-CDK12 antibody [EPR29009-30] (ab317746) at 1/1000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 164 kDa
Observed band size: 205 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDK12 with ab317746 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human stomach. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CDK12 with ab317746 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human testis. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling CDK12 with ab317746 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human colon carcinoma. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CDK12 with ab317746 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CDK12 with ab317746 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling CDK12 with ab317746 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat colon. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody [EPR29009-30] (ab317746)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling CDK12 with ab317746 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat testis. The section was incubated with ab317746 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-CDK12 antibody [EPR29009-30] (ab317746)

CDK12 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell fresh lysate with ab317746 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317746 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell fresh lysate
Lane 2: ab317746 IP in 293T (human embryonic kidney epithelial cell) whole cell fresh lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317746 in 293T whole cell fresh lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
Anti-CDK12 antibody [EPR29009-30] (ab317746)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

HRP detection kit

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

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