Anti-CDK11/CDC2L2/CDK11A抗体(ab19393)
Key features and details
- Rabbit polyclonal to CDK11/CDC2L2/CDK11A
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CDK11/CDC2L2/CDK11A抗体
参阅全部 CDK11/CDC2L2/CDK11A 一抗 -
描述
兔多克隆抗体to CDK11/CDC2L2/CDK11A -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to CDK11/CDC2L2/CDK11A immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19393于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| WB | (3) |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 90 kDa).
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| IHC-P |
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 90 kDa). |
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IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression. -
组织特异性
Expressed ubiquitously. Some evidence of isoform-specific tissue distribution. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
During apoptosis, induced by Fas or tumor necrosis factor, specific CKD11 p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the CDK11 proteins. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 728642 Human
- Entrez Gene: 12537 Mouse
- Omim: 116951 Human
- SwissProt: Q9UQ88 Human
- SwissProt: P24788 Mouse
- Unigene: 651228 Human
- Unigene: 709182 Human
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别名
- CD11A_HUMAN antibody
- CDC2L2 antibody
- CDC2L3 antibody
see all
图片
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Western blot - Anti-CDK11/CDC2L2/CDK11A antibody (ab19393)All lanes : Anti-CDK11/CDC2L2/CDK11A antibody (ab19393) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK11/CDC2L2/CDK11A antibody (ab19393)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human lung carcinoma tissue labelling CDK11/CDC2L2/CDK11A with ab19393 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-CDK11/CDC2L2/CDK11A antibody (ab19393)CDK11/CDC2L2/CDK11A was immunoprecipitated from HeLa whole cell lysate with 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab19393 at 1 µg/ml dilution.
Lane 1: ab19393 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab19393 被引用在 7 文献中.
- An S et al. A cyclin-dependent kinase, CDK11/p58, represses cap-dependent translation during mitosis. Cell Mol Life Sci 77:4693-4708 (2020). PubMed: 32030451
- Renshaw MJ et al. CDK11p58-cyclin L1ß regulates abscission site assembly. J Biol Chem 294:18639-18649 (2019). PubMed: 31653703
- Holmlund T et al. GCN5 acetylates and regulates the stability of the oncoprotein E2A-PBX1 in acute lymphoblastic leukemia. Leukemia 27:578-85 (2013). PubMed: 23044487
- Eldridge A et al. Manganese superoxide dismutase interacts with a large scale of cellular and mitochondrial proteins in low-dose radiation-induced adaptive radioprotection. Free Radic Biol Med 53:1838-47 (2012). WB . PubMed: 23000060
- Wilkinson S et al. The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. Autophagy 7:1295-301 (2011). WB . PubMed: 21808150
- Ohno S et al. Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11(p58) in mouse embryonic stem cells. Cell Cycle 10:3706-13 (2011). PubMed: 22037210
- Barna M et al. Suppression of Myc oncogenic activity by ribosomal protein haploinsufficiency. Nature 456:971-5 (2008). WB ; Mouse . PubMed: 19011615